Alignments for a candidate for actP in Streptacidiphilus oryzae TH49

GapMind for catabolism of small carbon sources

Alignments for a candidate for actP in Streptacidiphilus oryzae TH49

Align Monocarboxylic acid transporter (characterized)
to candidate WP_051939883.1 BS73_RS13175 cation acetate symporter

Query= SwissProt::Q8NS49
         (551 letters)



>NCBI__GCF_000744815.1:WP_051939883.1
          Length = 546

 Score =  516 bits (1330), Expect = e-151
 Identities = 276/554 (49%), Positives = 372/554 (67%), Gaps = 20/554 (3%)

Query: 1   MNSTILLAQDAVSEGVGNPILNISVFVVFIIVTMTVVLRVGKSTSESTDFYTGGASFSGT 60
           M++ +L A         +  L+ ++F   ++VT+ V +  G+    + ++YTGG  F+  
Sbjct: 1   MHANLLAAAGGGQPHNAHQALSTALFSAVVLVTLGVTVWAGRRVRAAEEYYTGGRQFTPM 60

Query: 61  QNGLAIAGDYLSAASFLGIVGAISLNGYDGFLYSIGFFVAWLVALLLVAEPLRNVGRFTM 120
           QNG AI+GDY+SAASFLG+ G I+L GYDG LY+IGF V WLV L+L+AE +RN GR+T+
Sbjct: 61  QNGFAISGDYMSAASFLGVTGLIALYGYDGLLYTIGFLVGWLVVLVLLAELVRNTGRYTL 120

Query: 121 ADVLSFRLRQKPVRVAAACGTLAVTLFYLIAQMAGAGSLVSVLLDIHEFKWQAVVVGIVG 180
           ADVLS R+RQ+PVR A    ++ V LFYL+AQM GAGS+V++LL  +    +A  +  VG
Sbjct: 121 ADVLSVRMRQRPVRAATGVASMVVALFYLLAQMVGAGSVVAMLLGQNTDAARAWSIVGVG 180

Query: 181 IVMIAYVLLGGMKGTTYVQMIKAVLLVGGVAIMTVLTFVKVSGGLTTLLNDAVEKHAASD 240
            +M  YV +GGM+ TT++Q+IKAVLL+GG  ++  L  ++  G ++ LL+     HAA  
Sbjct: 181 ALMTVYVAIGGMRATTWIQIIKAVLLLGGTVLLCALVLIRFHGNVSELLS-----HAARG 235

Query: 241 YAATKGYDPTQILEPGLQYGATLTTQLDFISLALALCLGTAGLPHVLMRFYTVPTAKEAR 300
                 Y     L PGL YG +   +LDF+SL LAL LGT GLPH+L RFYTVPTA+ AR
Sbjct: 236 SGFGDAY-----LRPGLTYGGSWQNRLDFVSLGLALVLGTGGLPHILSRFYTVPTARAAR 290

Query: 301 KSVTWAIVLIGAFYLMTLVLGYGAAALVGPDRVIAAPGAANAAAPLLAFEL-------GG 353
           +S  WAI L+G FYLM ++LG GAAALVG DRV A+  A N A PLLA  L       GG
Sbjct: 291 RSALWAIGLVGGFYLMMIILGLGAAALVGADRVRASNAAGNTAVPLLAQVLGGGAGSTGG 350

Query: 354 SIFMALISAVAFATVLAVVAGLAITASAAVGHDIYNAVIRNGQS---TEAEQVRVSRITV 410
           ++  A+I+AVAFATVLAVVAGL + +S A  HD++    R       +EA++V V+R   
Sbjct: 351 AVLFAVIAAVAFATVLAVVAGLTMASSVAFAHDLWARAFRRADEPTPSEADEVAVARGAA 410

Query: 411 VVIGLISIVLGILAMTQNVAFLVALAFAVAASANLPTILYSLYWKKFNTTGAVAAIYTGL 470
           V +G I+IVL + A   NVA LV LAFA+AASANLPT+L++L+W++F T GAV A Y GL
Sbjct: 411 VGVGAIAIVLSLFAQKLNVASLVGLAFAIAASANLPTLLFNLFWRRFTTRGAVWATYGGL 470

Query: 471 ISALLLIFLSPAVSGNDSAMVPGADWAIFPLKNPGLVSIPLAFIAGWIGTLVGKPDNMDD 530
           I ALLL+  SP VSG+ S++ PG D+  FPL+NPG+VSIP  F+AGW+GT+  +     D
Sbjct: 471 IPALLLVAFSPVVSGSPSSVFPGVDFHWFPLQNPGVVSIPCGFLAGWLGTVTSRRSADPD 530

Query: 531 LAAEMEVRSLTGVG 544
             AE+EVR+LTG G
Sbjct: 531 AYAELEVRALTGAG 544


Lambda     K      H
   0.324    0.138    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 796
Number of extensions: 40
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 551
Length of database: 546
Length adjustment: 36
Effective length of query: 515
Effective length of database: 510
Effective search space:   262650
Effective search space used:   262650
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources