Align L-fucose mutarotase (EC 5.1.3.29) (characterized)
to candidate WP_037577568.1 BS73_RS29745 L-rhamnose mutarotase
Query= reanno::Smeli:SM_b21108 (109 letters) >NCBI__GCF_000744815.1:WP_037577568.1 Length = 107 Score = 126 bits (316), Expect = 9e-35 Identities = 56/109 (51%), Positives = 70/109 (64%), Gaps = 2/109 (1%) Query: 1 MQRMGMVIGLEPSKIAEYKRLHAAVWPEILALISECNITNYSIFLKEPENLLFGYWEYVG 60 M+R ++ L P AEY+R+H VWP +L I CNI NYSIFL + +LF Y+EYVG Sbjct: 1 MERHAQIVRLRPEHEAEYRRIHQQVWPAVLRTIHACNIRNYSIFLND--GVLFAYYEYVG 58 Query: 61 EDFEADMTKMAAHPKNQEWWSVCMPCQKPLESRRQGEWWAMMEEVFHHD 109 ED ADM KMA P+ Q WW + P Q PL+ +G+WWA EEVFH D Sbjct: 59 EDHAADMAKMAEDPETQRWWKITDPMQSPLDGTPEGQWWAPAEEVFHTD 107 Lambda K H 0.321 0.134 0.456 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 75 Number of extensions: 2 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 109 Length of database: 107 Length adjustment: 12 Effective length of query: 97 Effective length of database: 95 Effective search space: 9215 Effective search space used: 9215 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.4 bits) S2: 40 (20.0 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory