GapMind for catabolism of small carbon sources

 

D-mannitol catabolism in Streptacidiphilus oryzae TH49

Best path

mtlE, mtlF, mtlG, mtlK, mt2d, scrK

Rules

Overview: Mannitol degradation in GapMind is based on MetaCyc pathway mannitol degradation I via a phosphotransferase system (link), pathway II via mannitol 1-dehydrogenase (link), or another oxidative pathway with mannitol 2-dehydrogenase (PMID:8254318).

17 steps (10 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
mtlE polyol ABC transporter, substrate-binding component MtlE/SmoE BS73_RS07825
mtlF polyol ABC transporter, permease component 1 (MtlF/SmoF) BS73_RS07830 BS73_RS30605
mtlG polyol ABC transporter, permease component 2 (MtlG/SmoG) BS73_RS07835 BS73_RS31740
mtlK polyol ABC transporter, ATP component MtlK/SmoG BS73_RS18100 BS73_RS14235
mt2d mannitol 2-dehydrogenase BS73_RS07840 BS73_RS11735
scrK fructokinase BS73_RS07575 BS73_RS28445
Alternative steps:
cmtA mannitol phosphotransferase system, EII-CB component CmtA/MtlF
cmtB mannitol phosphotransferase system, EII-A component CmtB/MtlF
gutA mannitol PTS system, EII-C2 component GutA
gutB mannitol PTS system, EII-A component GutB
gutE mannitol PTS system, EII-BC1 component GutE
mak mannose kinase BS73_RS28445 BS73_RS04690
manA mannose-6-phosphate isomerase BS73_RS24055 BS73_RS22910
mt1d mannitol 1-dehydrogenase BS73_RS27735 BS73_RS11045
mtlA mannitol phosphotransferase system, EII-CBA components
mtlD mannitol-1-phosphate 5-dehydrogenase
PLT5 polyol transporter PLT5 BS73_RS21865 BS73_RS21860

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory