Align Maltose transport system permease protein malG aka TT_C1629, component of The trehalose/maltose/sucrose/palatinose porter (TTC1627-9) plus MalK1 (ABC protein, shared with 3.A.1.1.24) (Silva et al. 2005; Chevance et al., 2006). The receptor (TTC1627) binds disaccharide alpha-glycosides, namely trehalose (alpha-1,1), sucrose (alpha-1,2), maltose (alpha-1,4), palatinose (alpha-1,6) and glucose (characterized)
to candidate WP_084704318.1 BS73_RS24195 carbohydrate ABC transporter permease
Query= TCDB::Q72H66 (280 letters) >NCBI__GCF_000744815.1:WP_084704318.1 Length = 300 Score = 228 bits (581), Expect = 1e-64 Identities = 119/266 (44%), Positives = 165/266 (62%), Gaps = 4/266 (1%) Query: 15 LLVVFVVVYSVFPFYWAVISSFKPSDALFSPDPSFLPVPFTLEHYENVFLQANFGRNLLN 74 L + V ++ P YW V SSFK + +P P T +Y N F Q F + N Sbjct: 39 LAALLVALFVALPLYWLVASSFKGDKEIGTPH--LYPHAATGANYSNAFSQYAFTTYIEN 96 Query: 75 SLIVAGGATLLSLVLGVLAAYALGRLPFPPKNAVMYIVLSMTMFPQIAVLGGLFLLLRQT 134 S I+A T+L L LG A YAL RLP K VM +L +++FP +A++ L+LLLR Sbjct: 97 SAIIAVSTTVLVLGLGTFAGYALARLPVRGKTGVMTALLMISLFPTVALVAPLYLLLRDI 156 Query: 135 GLFNTHLGLILTYLLFTLPFTVWVLVGYFRGLPRELEEAAYVDGATPLQTLLKVMLPLTG 194 G N++ GLIL Y TLPF +W+L YF +P+E+EE A+VDGA+P +T++ V+LP Sbjct: 157 GWLNSYQGLILAYTALTLPFAIWILRNYFLSIPKEMEEVAWVDGASPARTVVSVILPQAL 216 Query: 195 PGLVTTGLLAFIAAWNEYLFALTFTVGDSVKTVPPAIASFGGATPFEIPWGSIMAASVVV 254 PGL T G+ F+AAW E+L AL+ D +T+P IA FGG F P+G+I AAS V Sbjct: 217 PGLFTAGVFTFVAAWTEFLIALSVNSADRYRTIPVGIALFGG--QFVTPYGTIFAASAVA 274 Query: 255 TVPLVVLVLVFQQRIVAGLTAGAVKG 280 +P+ +LVL+F++ +V+GLTAGAVKG Sbjct: 275 MLPIALLVLIFRRAVVSGLTAGAVKG 300 Lambda K H 0.329 0.145 0.439 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 176 Number of extensions: 8 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 280 Length of database: 300 Length adjustment: 26 Effective length of query: 254 Effective length of database: 274 Effective search space: 69596 Effective search space used: 69596 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory