GapMind for catabolism of small carbon sources

 

Alignments for a candidate for natA in Streptacidiphilus oryzae TH49

Align NatA, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (characterized)
to candidate WP_037577453.1 BS73_RS29115 ABC transporter ATP-binding protein

Query= TCDB::Q7A2H0
         (260 letters)



>NCBI__GCF_000744815.1:WP_037577453.1
          Length = 298

 Score =  181 bits (458), Expect = 2e-50
 Identities = 103/253 (40%), Positives = 150/253 (59%), Gaps = 8/253 (3%)

Query: 10  PLLAASGLCKSFGGIKAVQEARIEVAQGSITGLIGPNGAGKTTLFNLLSNFIRPDKGRVI 69
           PLL A  +   FGG+ AV +  + V  G I GLIGPNGAGKTT FN L+    P +GRV 
Sbjct: 18  PLLEADHVTMRFGGLTAVNDVSLGVRPGEILGLIGPNGAGKTTFFNCLTGLYVPTEGRVS 77

Query: 70  FDGEPIQQLQPHQIAQQGMVRTFQVARTLSRLSVLENMLLAAQKQTGENFWQVQLQPQVV 129
           + G  +   +P ++ Q G+ RTFQ  R  + ++VLEN+L+    +T E  +   ++    
Sbjct: 78  YRGRTLPP-KPFKVTQAGIARTFQNIRLFNNMTVLENVLVGRHTRTREGLFSALVRGPGY 136

Query: 130 VKEEKQLQEQAMFLLESVGLAKKAYEYAGGLSGGQRKLLEMGRALMTNPKLILLDEPAAG 189
            + E++ +  AM +LE VGL  KA   A  L  G+++ LE+ RAL + P L+LLDEP AG
Sbjct: 137 RRAERESRRHAMEILEFVGLEAKAEHLARNLPYGEQRKLEIARALASEPGLLLLDEPTAG 196

Query: 190 VNP---RLIDDICDRILTWNRQDGMTFLIIEHNMDVIMSLCDRVWVLAEGQNLADGTPAE 246
           +NP   R  +++   I    R  G+  L+IEH+M  I +LCDRV VL +GQ L +G+   
Sbjct: 197 MNPQETRATEELVFAI----RARGIAVLVIEHDMRFIFNLCDRVAVLVQGQLLVEGSSET 252

Query: 247 IQTNSQVLEAYLG 259
           +Q + +V+ AYLG
Sbjct: 253 VQADERVIAAYLG 265


Lambda     K      H
   0.319    0.136    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 176
Number of extensions: 12
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 260
Length of database: 298
Length adjustment: 25
Effective length of query: 235
Effective length of database: 273
Effective search space:    64155
Effective search space used:    64155
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory