Align Succinate-acetate/proton symporter SatP; Succinate-acetate transporter protein (characterized)
to candidate WP_048082643.1 EI99_RS14425 hypothetical protein
Query= SwissProt::P0AC98 (188 letters) >NCBI__GCF_000746075.1:WP_048082643.1 Length = 199 Score = 208 bits (530), Expect = 4e-59 Identities = 102/177 (57%), Positives = 129/177 (72%) Query: 7 ANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIFYGGIAQIFAGLLEYKKGNTFGL 66 ANPAPLGL+ FG+TT+LLN HN G++ L+ +ILAMG YGGIAQI A L+EY+ GNTF + Sbjct: 22 ANPAPLGLIAFGLTTVLLNFHNAGFYELNSMILAMGFAYGGIAQIIASLMEYRNGNTFAM 81 Query: 67 TAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVYLGLWGVFTLFMFFGTLKGARVLQF 126 AF SYG FW + V +L++PKL L AP+A L YL +WG+FTL MF GTLK +R LQ Sbjct: 82 VAFGSYGLFWWSFVFLLIIPKLNLGAAPDAVSLAAYLFMWGLFTLVMFVGTLKISRGLQV 141 Query: 127 VFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLICGASAIYLAMGEVLNEQFGRTVLP 183 VF SL +LF LL+ G I GN+ I AG+ G+ G SAIY+ + EVLNE +G+ VLP Sbjct: 142 VFLSLAILFFLLSAGEITGNSMITLIAGYEGIFTGFSAIYVGLAEVLNELYGKKVLP 198 Lambda K H 0.330 0.147 0.457 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 181 Number of extensions: 5 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 188 Length of database: 199 Length adjustment: 20 Effective length of query: 168 Effective length of database: 179 Effective search space: 30072 Effective search space used: 30072 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 44 (21.6 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory