GapMind for catabolism of small carbon sources

 

Protein WP_034211290.1 in Arenimonas metalli CF5-1

Annotation: NCBI__GCF_000747155.1:WP_034211290.1

Length: 426 amino acids

Source: GCF_000747155.1 in NCBI

Candidate for 5 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-malate catabolism dctM lo C4-dicarboxylate TRAP transporter large permease protein DctM (characterized) 32% 95% 259.2 Dicarboxylate transporter (DctM), component of TRAP transporter for a hydrophobic substrate (3-d structure known; tp0958 has 18-20 TMSs) (Deka et al., 2012). The substrate could be a lipoprotein, tp0956 (O83922) which is encoded in the same operon with tp0957 and tp058. This protein differs from all other members of the TRAP-T family in having 19 predicted TMSs with extra TMSs at its N-terminus 40% 301.6
fumarate catabolism dctM lo C4-dicarboxylate TRAP transporter large permease protein DctM (characterized) 32% 95% 259.2 Dicarboxylate transporter (DctM), component of TRAP transporter for a hydrophobic substrate (3-d structure known; tp0958 has 18-20 TMSs) (Deka et al., 2012). The substrate could be a lipoprotein, tp0956 (O83922) which is encoded in the same operon with tp0957 and tp058. This protein differs from all other members of the TRAP-T family in having 19 predicted TMSs with extra TMSs at its N-terminus 40% 301.6
succinate catabolism dctM lo C4-dicarboxylate TRAP transporter large permease protein DctM (characterized) 32% 95% 259.2 Dicarboxylate transporter (DctM), component of TRAP transporter for a hydrophobic substrate (3-d structure known; tp0958 has 18-20 TMSs) (Deka et al., 2012). The substrate could be a lipoprotein, tp0956 (O83922) which is encoded in the same operon with tp0957 and tp058. This protein differs from all other members of the TRAP-T family in having 19 predicted TMSs with extra TMSs at its N-terminus 40% 301.6
D-glucuronate catabolism dctM lo Putative TRAP dicarboxylate transporter, DctM subunit (characterized, see rationale) 35% 97% 243.8 Dicarboxylate transporter (DctM), component of TRAP transporter for a hydrophobic substrate (3-d structure known; tp0958 has 18-20 TMSs) (Deka et al., 2012). The substrate could be a lipoprotein, tp0956 (O83922) which is encoded in the same operon with tp0957 and tp058. This protein differs from all other members of the TRAP-T family in having 19 predicted TMSs with extra TMSs at its N-terminus 40% 301.6
D-gluconate catabolism gntB lo D-gluconate TRAP transporter, large permease component (characterized) 33% 98% 226.1 Dicarboxylate transporter (DctM), component of TRAP transporter for a hydrophobic substrate (3-d structure known; tp0958 has 18-20 TMSs) (Deka et al., 2012). The substrate could be a lipoprotein, tp0956 (O83922) which is encoded in the same operon with tp0957 and tp058. This protein differs from all other members of the TRAP-T family in having 19 predicted TMSs with extra TMSs at its N-terminus 40% 301.6

Sequence Analysis Tools

View WP_034211290.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MSLGLGLAIILGLAALGMPLFAVIAAIALLGFHLAGYDLIAVAVEFYRLGDMPGLIAIPL
FTLAGYLLGESQAPKRLVRLSNALLGWLPGGLAIVAIVASAFFTAFTGASGVTIVALGAL
LYPALRQAGYGERYSLGLVTAAGSLGLLFAPSLPLILYGFVAGQMGSDPPVTIPDLFLAG
LLPGLLMVGMLSLHAMWVGRGIPQSRHRFDTAELARALRETAWELPLPVIVLGGIYTGAL
AASEAAAVTALYVLVVTVLIKKEISFRKLPEVMAESMRLVGAILLILGAALALSNWLVDQ
EVPATLFAALSEHVDSPWVFLLLLNLFLLVVGMLLDIFSAVVILTPLLLPVAAGFGIHPV
HLGVVMLANLQLGYFTPPVGMNLFIAAYRFGQPVLVLVRACVPFFLVLAAAVALITWWPA
LSLGLL

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory