GapMind for catabolism of small carbon sources

 

Protein WP_038018191.1 in Tatumella morbirosei LMG 23360

Annotation: NCBI__GCF_000757425.2:WP_038018191.1

Length: 324 amino acids

Source: GCF_000757425.2 in NCBI

Candidate for 14 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
D-cellobiose catabolism manX hi PTS system mannose-specific EIIAB component; EC 2.7.1.-; EC 2.7.1.69 (characterized) 86% 100% 547.4
D-fructose catabolism levDE hi PTS system mannose-specific EIIAB component; EC 2.7.1.-; EC 2.7.1.69 (characterized) 86% 100% 547.4 PTS system, mannose-specific, IIAB component, component of The primary glucose /mannose uptake transporter, ManLMN 40% 251.9
D-glucosamine (chitosamine) catabolism manX hi PTS system mannose-specific EIIAB component; EC 2.7.1.-; EC 2.7.1.69 (characterized) 86% 100% 547.4 PTS system, mannose-specific, IIAB component, component of The primary glucose /mannose uptake transporter, ManLMN 40% 251.9
D-glucose catabolism manX hi PTS system mannose-specific EIIAB component; EC 2.7.1.-; EC 2.7.1.69 (characterized) 86% 100% 547.4
lactose catabolism manX hi PTS system mannose-specific EIIAB component; EC 2.7.1.-; EC 2.7.1.69 (characterized) 86% 100% 547.4
D-maltose catabolism manX hi PTS system mannose-specific EIIAB component; EC 2.7.1.-; EC 2.7.1.69 (characterized) 86% 100% 547.4
D-mannose catabolism manX hi PTS system mannose-specific EIIAB component; EC 2.7.1.-; EC 2.7.1.69 (characterized) 86% 100% 547.4
sucrose catabolism levDE hi PTS system mannose-specific EIIAB component; EC 2.7.1.-; EC 2.7.1.69 (characterized) 86% 100% 547.4 PTS system, mannose-specific, IIAB component, component of The primary glucose /mannose uptake transporter, ManLMN 40% 251.9
sucrose catabolism manX hi PTS system mannose-specific EIIAB component; EC 2.7.1.-; EC 2.7.1.69 (characterized) 86% 100% 547.4
trehalose catabolism manX hi PTS system mannose-specific EIIAB component; EC 2.7.1.-; EC 2.7.1.69 (characterized) 86% 100% 547.4
D-fructose catabolism levE med PTS system fructose-specific EIIB component; EIIB-Fru; Fructose-specific phosphotransferase enzyme IIB component; lev-PTS; p18; EC 2.7.1.202 (characterized) 46% 99% 156 PTS system mannose-specific EIIAB component; EC 2.7.1.-; EC 2.7.1.69 86% 547.4
sucrose catabolism levE med PTS system fructose-specific EIIB component; EIIB-Fru; Fructose-specific phosphotransferase enzyme IIB component; lev-PTS; p18; EC 2.7.1.202 (characterized) 46% 99% 156 PTS system mannose-specific EIIAB component; EC 2.7.1.-; EC 2.7.1.69 86% 547.4
D-fructose catabolism levD lo PTS system fructose-specific EIIA component; EIIA-Fru; Fructose-specific phosphotransferase enzyme IIA component; lev-PTS; p16 (characterized) 34% 83% 80.5 PTS system mannose-specific EIIAB component; EC 2.7.1.-; EC 2.7.1.69 86% 547.4
sucrose catabolism levD lo PTS system fructose-specific EIIA component; EIIA-Fru; Fructose-specific phosphotransferase enzyme IIA component; lev-PTS; p16 (characterized) 34% 83% 80.5 PTS system mannose-specific EIIAB component; EC 2.7.1.-; EC 2.7.1.69 86% 547.4

Sequence Analysis Tools

View WP_038018191.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MTIAIVIGTHGWAAEQLLKTSEMLLGEQGNVGWIDFVPGENADTLIEKYTARLTELDTSE
GVLFLVDTWGGSPFNAASRIVVDKENYEVIAGVNVPMLVETFMARDDNPTFAELVALALE
TGREGVRALKHQPEEAAAPVAAPQPAKPAVAAPAKPMSASDYMVIGLARIDDRLIHGQVA
TRWTKETNVTRIIVVSDEVAADQVRKTLLTQVAPPGVTAHVVDIAKMLRVYNNPKYAGQR
VMLLFTNPTDVERVVEGGVKITSVNIGGMAFRQGKTQVNNAVSVDQKDIEAFKKLNARGI
ELEVRKVSSDQKLKMMDLIGKVSQ

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory