Annotation: NCBI__GCF_000757425.2:WP_046791705.1
Length: 473 amino acids
Source: GCF_000757425.2 in NCBI
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|---|---|---|---|---|---|---|---|---|
| D-cellobiose catabolism | bgl | hi | β-glucosidase (BglY) (EC 3.2.1.21) (characterized) | 71% | 100% | 725.7 | Thit_0299 (EC 3.2.1.-) | 64% | 635.2 |
| D-cellobiose catabolism | ascB | med | 6-phospho-β-glycosidase (GK3214) (EC 3.2.1.85|3.2.1.86) (characterized) | 49% | 100% | 463.4 | β-glucosidase (BglY) (EC 3.2.1.21) | 71% | 725.7 |
| lactose catabolism | pbgal | med | 6-phospho-β-glycosidase (GK3214) (EC 3.2.1.85|3.2.1.86) (characterized) | 49% | 100% | 463.4 | β-glucosidase (BglY) (EC 3.2.1.21) | 71% | 725.7 |
| lactose catabolism | lacZ | med | β-galactosidase (GKGal1A;GK2337) (Gal1A) (EC 3.2.1.23) (characterized) | 44% | 99% | 402.1 | β-glucosidase (BglY) (EC 3.2.1.21) | 71% | 725.7 |
View WP_046791705.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
MKYSKLKPFPSGFLWGASTSAYQIEGAWNEEGKGPSVIDMRHEYPQGTTDFKVASDHYHQ YKQDIRLLSETGLKAYRFSIAWTRIFPAGDGEINPAGLQFYHNLIDEIRSCGMEPIVTLY HFDLPQTLQDIGGWHNRRTVDAFIRYANTLFTELGSKVKYWLTINEQNLMILQGNALGTV DPKVQNNKKHLYQQNHNMLVAQAAVMENLHKNHPEALIGPAPNIALIYAASPKPEDVLAS ENYNAIRNWLYLDMAVSGQYNHLAWAYLEEKGYLPEIEEGDMEILSRGKPDFIAFNYYTT QTVEASRGDGHDEVIRGGNKYLKSGEDGVHRGASNNFLPRTEFGTEIDPVGFRNTLRAIY GRYHLPLLITENGIGAFDKPDADGTINDQYRIDYLRSHIEQMQLAITDGVRIIGYTPWSA IDLISTHQGCSKRYGLIYVNRDEFDLRDLKRIRKKSSFWYEKVILNNGIDGID
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory