GapMind for catabolism of small carbon sources

 

Alignments for a candidate for crr in Tatumella morbirosei LMG 23360

Align Putative PTS system sugar phosphotransferase component IIA (characterized, see rationale)
to candidate WP_038022033.1 HA49_RS08410 PTS glucose transporter subunit IIA

Query= uniprot:Q9KZP2
         (149 letters)



>NCBI__GCF_000757425.2:WP_038022033.1
          Length = 167

 Score = 88.2 bits (217), Expect = 6e-23
 Identities = 53/143 (37%), Positives = 79/143 (55%), Gaps = 5/143 (3%)

Query: 4   VSSPLAGRAIGLAAVPDPVFSGAMVGPGTAIDPVREPSEAVAPVDGVIVSLHP--HAFVV 61
           + +PL+G  + +  VPD VF+  +VG G AI P    S  VAPVDG I  +    HAF +
Sbjct: 21  IVAPLSGEIVNIEDVPDVVFAEKIVGDGVAIRP--NGSTMVAPVDGTIGKIFETNHAFSI 78

Query: 62  VDESGHGVLTHLGIDTVQLNGEGFELLVNKGDTVVRGQGVVRWDPAAVEAAGKSPICPIV 121
             ++G  +  H GIDTV+L GEGF  + ++G  V +G  V+ +D   +E   KS + P+V
Sbjct: 79  ESDNGVELFVHFGIDTVELKGEGFRRIASEGQKVKKGDIVIEFDLPLLEEKAKSTLTPVV 138

Query: 122 ALEATAEALADLREDGDVKAGES 144
            +    E    ++  G V AGE+
Sbjct: 139 -ISNMDEIKELVKLTGQVVAGET 160


Lambda     K      H
   0.316    0.136    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 78
Number of extensions: 6
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 149
Length of database: 167
Length adjustment: 17
Effective length of query: 132
Effective length of database: 150
Effective search space:    19800
Effective search space used:    19800
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 43 (21.2 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory