GapMind for catabolism of small carbon sources

 

Alignments for a candidate for galdh in Tatumella morbirosei LMG 23360

Align L-arabinose 1-dehydrogenase; D-galactose 1-dehydrogenase (EC 1.1.1.46; EC 1.1.1.48) (characterized)
to candidate WP_038017963.1 HA49_RS12580 gluconate 5-dehydrogenase

Query= reanno::BFirm:BPHYT_RS16920
         (266 letters)



>NCBI__GCF_000757425.2:WP_038017963.1
          Length = 254

 Score =  101 bits (251), Expect = 2e-26
 Identities = 74/243 (30%), Positives = 105/243 (43%)

Query: 22  LVDRTVLITGGATGIGASFVEHFAAQGARVAFFDIDASAGEALADELGDSKHKPLFLSCD 81
           L  R  LITG   G+G          GA V     D    E    EL     +    + D
Sbjct: 7   LTGRRALITGSTQGLGLLMARGLGEHGAEVIINGRDPQRCENAVAELQALGIRASARAFD 66

Query: 82  LTDIDALQKAIADVKAALGPIQVLVNNAANDKRHTIGEVTRESFDAGIAVNIRHQFFAAQ 141
           +TD  A+  AI  ++   GP+ +L+NNA    R    E   E +D  I VN +  F  +Q
Sbjct: 67  VTDPVAVTAAIDSIETETGPLDILINNAGIQHRAPFTEFPVEQWDRIIDVNQKSVFLVSQ 126

Query: 142 AVMEDMKAANSGSIINLGSISWMLKNGGYPVYVMSKSAVQGLTRGLARDLGHFNIRVNTL 201
            V   M     G IIN+ S+   L       Y  SK AV+ LTRG+  +L  +NI+VN +
Sbjct: 127 QVARKMMVRKRGKIINICSMQSELGRDNITPYAASKGAVKMLTRGMCVELARYNIQVNAI 186

Query: 202 VPGWVMTEKQKRLWLDDAGRRSIKEGQCIDAELEPADLARMALFLAADDSRMITAQDIVV 261
            PG+  +E    L  D      + +        +P +L   A+FL+A  S  +    + V
Sbjct: 187 APGYFDSEMTAALVNDKQFSDWLCQRTPAARWGKPEELMGAAVFLSATASDFVNGHVLFV 246

Query: 262 DGG 264
           DGG
Sbjct: 247 DGG 249


Lambda     K      H
   0.320    0.134    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 152
Number of extensions: 10
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 266
Length of database: 254
Length adjustment: 24
Effective length of query: 242
Effective length of database: 230
Effective search space:    55660
Effective search space used:    55660
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory