GapMind for catabolism of small carbon sources

 

Protein WP_043532085.1 in Halomonas xinjiangensis TRM 0175

Annotation: NCBI__GCF_000759345.1:WP_043532085.1

Length: 263 amino acids

Source: GCF_000759345.1 in NCBI

Candidate for 19 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
phenylacetate catabolism paaG hi 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) (characterized) 67% 96% 333.2 Trans-2-decenoyl-[acyl-carrier-protein] isomerase; EC 5.3.3.14 37% 163.7
L-phenylalanine catabolism paaG hi 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) (characterized) 67% 96% 333.2 Trans-2-decenoyl-[acyl-carrier-protein] isomerase; EC 5.3.3.14 37% 163.7
L-isoleucine catabolism ech med Probable enoyl-CoA hydratase; EC 4.2.1.17 (uncharacterized) 48% 100% 231.9 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) 67% 333.2
L-isoleucine catabolism ech med paaB: phenylacetate degradation probable enoyl-CoA hydratase PaaB (EC 4.2.1.17) (TIGR02280) 99% 411.6 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) 67% 333.2
4-hydroxybenzoate catabolism badK lo BadK (characterized) 37% 95% 163.3 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) 67% 333.2
phenylacetate catabolism badK lo BadK (characterized) 37% 95% 163.3 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) 67% 333.2
L-phenylalanine catabolism badK lo BadK (characterized) 37% 95% 163.3 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) 67% 333.2
4-hydroxybenzoate catabolism ech lo trans-2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) (characterized) 34% 100% 157.1 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) 67% 333.2
4-hydroxybenzoate catabolism paaF lo trans-2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) (characterized) 34% 100% 157.1 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) 67% 333.2
L-arginine catabolism ech lo trans-2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) (characterized) 34% 100% 157.1 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) 67% 333.2
L-citrulline catabolism ech lo trans-2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) (characterized) 34% 100% 157.1 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) 67% 333.2
L-lysine catabolism ech lo trans-2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) (characterized) 34% 100% 157.1 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) 67% 333.2
phenylacetate catabolism ech lo trans-2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) (characterized) 34% 100% 157.1 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) 67% 333.2
phenylacetate catabolism paaF lo trans-2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) (characterized) 34% 100% 157.1 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) 67% 333.2
L-phenylalanine catabolism ech lo trans-2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) (characterized) 34% 100% 157.1 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) 67% 333.2
L-phenylalanine catabolism paaF lo trans-2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) (characterized) 34% 100% 157.1 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) 67% 333.2
L-proline catabolism ech lo trans-2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) (characterized) 34% 100% 157.1 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) 67% 333.2
L-valine catabolism ech lo trans-2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) (characterized) 34% 100% 157.1 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) 67% 333.2
phenylacetate catabolism paaZ1 lo Enoyl-CoA hydratase; EC 4.2.1.17 (characterized, see rationale) 32% 95% 120.9 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) 67% 333.2
L-phenylalanine catabolism paaZ1 lo Enoyl-CoA hydratase; EC 4.2.1.17 (characterized, see rationale) 32% 95% 120.9 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) 67% 333.2

Sequence Analysis Tools

View WP_043532085.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

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Sequence

MKFETIDFSILDGVAVLTLNRPKTLNSFNVQMHEEVNAALDRVDAENKVRCLLLTGHGRG
FSTGQDLSERKFGPYDEAPDLGHSLEVYYNPLIRRLRALQIPVLCAVNGVAAGAGANLAL
NCDIVLAARSASFVQAFCKLGLVPDSGGTWLLPRLVGRARARGLALLGDKLSAEQAEAWG
MIWKVVDDEALMEDALALARHLATQPTKGLAYIKQALDAAEDNDMNTQLDLERDLQKKAG
GSADYREGVAAFKEKRSPVFSGK

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory