GapMind for catabolism of small carbon sources

 

Alignments for a candidate for artM in Halomonas xinjiangensis TRM 0175

Align Amino acid (Lysine/arginine/ornithine/histidine/octopine) ABC transporter membrane protein, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR (characterized)
to candidate WP_043531902.1 JH15_RS15195 amino acid ABC transporter permease

Query= TCDB::Q9HU29
         (230 letters)



>NCBI__GCF_000759345.1:WP_043531902.1
          Length = 222

 Score =  152 bits (383), Expect = 7e-42
 Identities = 85/216 (39%), Positives = 132/216 (61%), Gaps = 9/216 (4%)

Query: 5   ELILKWMPKMLQGAALTLELLAIAVVAGLALALPLGIARASRHWYVRAVPYAYIFFFRGT 64
           E  ++W+P++LQG  +TL L  IA+  G  L L L  AR   +     +  AY+  FRGT
Sbjct: 6   EFGIEWLPRLLQGVPVTLLLWIIAITLGFFLGLMLCWARVYGNRLFYWISTAYVELFRGT 65

Query: 65  PLLLQLFIVYYGLAQFEEVRKSAFWPYLRDPYWCALLTMTLHTAAYIAEILRGAIHSVPV 124
           P+L+Q+F +Y GL     V          D +  A++ +TL++AAY AE  RG++ SVP 
Sbjct: 66  PMLVQMFFIYLGLPHVGIVF---------DAFTAAVIAITLNSAAYQAEYFRGSVLSVPK 116

Query: 125 GEVEAARALGMSRRQALWHIILPRAVRIGLPAYSNEVILMLKASAVVYTVTLFDIMGMAR 184
           G++ AARA GMS+ QA+ HI+LP+A+R  +P +SNE I+ LK +++ YT+ + +I  +A 
Sbjct: 117 GQLVAARACGMSQWQAIRHIMLPQALRRVIPQWSNEAIIELKFTSIAYTIGVVEITAIAS 176

Query: 185 TIIARTYESMLFFCLAGALYLVITIVLTRIFRLIER 220
            I +RT++    F  AG +YLV+T  +  +  L+ER
Sbjct: 177 DIGSRTFDFFQVFLWAGIIYLVLTSTVASLLGLLER 212


Lambda     K      H
   0.332    0.142    0.443 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 172
Number of extensions: 7
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 230
Length of database: 222
Length adjustment: 22
Effective length of query: 208
Effective length of database: 200
Effective search space:    41600
Effective search space used:    41600
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (22.0 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory