GapMind for catabolism of small carbon sources

 

Alignments for a candidate for hisP in Halomonas xinjiangensis TRM 0175

Align Probable ATP-binding component of ABC transporter, component of Amino acid transporter, PA5152-PA5155. Probably transports numerous amino acids including lysine, arginine, histidine, D-alanine and D-valine (Johnson et al. 2008). Regulated by ArgR (characterized)
to candidate WP_043532174.1 JH15_RS16045 ATP-binding cassette domain-containing protein

Query= TCDB::Q9HU32
         (257 letters)



>NCBI__GCF_000759345.1:WP_043532174.1
          Length = 262

 Score =  319 bits (818), Expect = 3e-92
 Identities = 158/251 (62%), Positives = 202/251 (80%)

Query: 5   TPALEIRNLHKRYGDLEVLKGISLTARDGDVISILGSSGSGKSTFLRCINLLENPHQGQI 64
           T A++ R+L K YG++ VL  +SL+  D  V+SI+GSSGSGKST LRC+NLLE P +G++
Sbjct: 10  TLAVDARHLQKHYGEVPVLHDVSLSVTDRHVVSIIGSSGSGKSTLLRCMNLLEIPDRGEL 69

Query: 65  LVSGEELRLKKSKNGDLVAADSQQINRLRSELGFVFQNFNLWPHMSILDNVIEAPRRVLG 124
            ++ E +R +++  GD V  D +Q+++LRS++  VFQ FNLWPH+S+L NVIEAP RV G
Sbjct: 70  TIANEAIRFQRNAQGDNVGIDRRQVHKLRSQVSMVFQQFNLWPHLSVLGNVIEAPLRVHG 129

Query: 125 KSKAEAIEIAEGLLAKVGIADKRHSYPAQLSGGQQQRAAIARTLAMQPKVILFDEPTSAL 184
            S+ EAIE  E  LAKVG+ADKR+++P+ LSGGQQQR AIAR LAM+P+V+LFDEPTSAL
Sbjct: 130 LSRREAIERGEHYLAKVGLADKRNAFPSFLSGGQQQRVAIARALAMEPRVLLFDEPTSAL 189

Query: 185 DPEMVQEVLNVIRALAEEGRTMLLVTHEMSFARQVSSEVVFLHQGLVEEQGTPQQVFENP 244
           DPE+V EVL VI  LA EGRTMLLVTHEMSFAR+VS+E+VFLHQG VEE G+PQQVFE  
Sbjct: 190 DPELVNEVLGVIGGLAGEGRTMLLVTHEMSFAREVSNEIVFLHQGHVEEAGSPQQVFEQA 249

Query: 245 QSARCKQFMSS 255
           +S RC+QF+SS
Sbjct: 250 RSDRCRQFLSS 260


Lambda     K      H
   0.317    0.133    0.367 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 199
Number of extensions: 3
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 257
Length of database: 262
Length adjustment: 24
Effective length of query: 233
Effective length of database: 238
Effective search space:    55454
Effective search space used:    55454
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory