GapMind for catabolism of small carbon sources

 

Alignments for a candidate for natA in Halomonas xinjiangensis TRM 0175

Align NatA, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (characterized)
to candidate WP_043531489.1 JH15_RS14395 ABC transporter ATP-binding protein

Query= TCDB::Q7A2H0
         (260 letters)



>NCBI__GCF_000759345.1:WP_043531489.1
          Length = 256

 Score =  225 bits (573), Expect = 8e-64
 Identities = 108/241 (44%), Positives = 166/241 (68%)

Query: 19  KSFGGIKAVQEARIEVAQGSITGLIGPNGAGKTTLFNLLSNFIRPDKGRVIFDGEPIQQL 78
           K+FGG+  + +  I+V +GSITG+IGPNGAGK+TLFN+++  +  D GRV+ DGE I  L
Sbjct: 12  KAFGGLTVIDDCSIQVERGSITGMIGPNGAGKSTLFNIIAGTLPLDSGRVLLDGEDITSL 71

Query: 79  QPHQIAQQGMVRTFQVARTLSRLSVLENMLLAAQKQTGENFWQVQLQPQVVVKEEKQLQE 138
               +  +G++RTFQ+A   S ++ LEN+++   +Q GE+ +    +P  V ++E +++ 
Sbjct: 72  PADVLFHKGLLRTFQIAHEFSHMTALENLMMVPPRQEGESLFSAWFKPGEVRRQEDEVRR 131

Query: 139 QAMFLLESVGLAKKAYEYAGGLSGGQRKLLEMGRALMTNPKLILLDEPAAGVNPRLIDDI 198
           +A+ +++ +GLA    E AG LSGGQ+KLLE+GR +MT  +++LLDE AAGVN  L+ D+
Sbjct: 132 RALEVIDFIGLAHVRNELAGNLSGGQKKLLELGRTMMTEARVVLLDEIAAGVNRTLLGDL 191

Query: 199 CDRILTWNRQDGMTFLIIEHNMDVIMSLCDRVWVLAEGQNLADGTPAEIQTNSQVLEAYL 258
              I   NR+ G TFL+IEH+MD+I  LCD V VLA+G  + +G+  ++Q N +V+EAY 
Sbjct: 192 MTNIERLNRELGYTFLVIEHDMDMIARLCDPVIVLAQGSVMMEGSIKDVQNNPEVIEAYF 251

Query: 259 G 259
           G
Sbjct: 252 G 252


Lambda     K      H
   0.319    0.136    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 186
Number of extensions: 4
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 260
Length of database: 256
Length adjustment: 24
Effective length of query: 236
Effective length of database: 232
Effective search space:    54752
Effective search space used:    54752
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory