GapMind for catabolism of small carbon sources

 

Alignments for a candidate for acnD in Halomonas xinjiangensis TRM 0175

Align 2-methylcitrate dehydratase (2-methyl-trans-aconitate forming) (EC 4.2.1.117) (characterized)
to candidate WP_043530831.1 JH15_RS12220 Fe/S-dependent 2-methylisocitrate dehydratase AcnD

Query= BRENDA::Q8EJW3
         (867 letters)



>NCBI__GCF_000759345.1:WP_043530831.1
          Length = 866

 Score = 1412 bits (3656), Expect = 0.0
 Identities = 695/861 (80%), Positives = 767/861 (89%)

Query: 5   MNTQYRKPLPGTALDYFDTREAIEAIAPGAYAKLPYTSRVLAENLVRRCEPEMLTASLKQ 64
           MNT++RKPLPGT+LD+FDTR A+EAI PGAY +LPYTSRVLAENLVRRC+P  LT SLKQ
Sbjct: 1   MNTEFRKPLPGTSLDHFDTRAAVEAILPGAYDRLPYTSRVLAENLVRRCDPAALTDSLKQ 60

Query: 65  IIESKQELDFPWFPARVVCHDILGQTALVDLAGLRDAIAAKGGDPAQVNPVVPTQLIVDH 124
           I+E K++LDFPW+PARVVCHDILGQTALVDLAGLRDAIA KGGDPA+VNPVVPTQLIVDH
Sbjct: 61  IVERKRDLDFPWYPARVVCHDILGQTALVDLAGLRDAIAEKGGDPAKVNPVVPTQLIVDH 120

Query: 125 SLAVEYGGFDKDAFAKNRAIEDRRNEDRFHFINWTQKAFKNIDVIPQGNGIMHQINLERM 184
           SLAVE+ GF+ DAF KNRAIEDRRN+DRFHFINWT+ AF+N+DVIP GNGIMHQINLE+M
Sbjct: 121 SLAVEHAGFESDAFEKNRAIEDRRNDDRFHFINWTKLAFENVDVIPPGNGIMHQINLEKM 180

Query: 185 SPVIHARNGVAFPDTLVGTDSHTPHVDALGVIAIGVGGLEAESVMLGRASYMRLPDIIGV 244
           SPV+  R+GVAFPDT VGTDSHTP VDALGVI++GVGGLEAESVMLGRAS MRLPDI+GV
Sbjct: 181 SPVVQKRDGVAFPDTCVGTDSHTPMVDALGVISVGVGGLEAESVMLGRASMMRLPDIVGV 240

Query: 245 ELTGKPQPGITATDIVLALTEFLRAQKVVSSYLEFFGEGAEALTLGDRATISNMTPEFGA 304
           ELTG+ QPGIT+TD+VLALT+FLR ++VV +YLEFFGEGA ALT+GDRATISNMTPE+GA
Sbjct: 241 ELTGRLQPGITSTDMVLALTDFLRRERVVGAYLEFFGEGARALTVGDRATISNMTPEYGA 300

Query: 305 TAAMFYIDQQTLDYLTLTGREAEQVKLVETYAKTAGLWSDDLKQAVYPRTLHFDLSSVVR 364
           TAAMFYID+QT+DYL LTGR+ +QV LVETYAK AGLW+D L  A Y R L FDLSSV R
Sbjct: 301 TAAMFYIDEQTIDYLKLTGRDEDQVTLVETYAKEAGLWADCLADADYERVLTFDLSSVPR 360

Query: 365 TIAGPSNPHARVPTSELAARGISGEVENEPGLMPDGAVIIAAITSCTNTSNPRNVIAAGL 424
           T+AGPSNPHA +PT+ELA RGISG V+++ G MPDGAVIIAAITSCTNTSNPRN++AAGL
Sbjct: 361 TLAGPSNPHALLPTAELAKRGISGNVQSDEGRMPDGAVIIAAITSCTNTSNPRNMVAAGL 420

Query: 425 LARNANAKGLTRKPWVKTSLAPGSKAVQLYLEEANLLPELESLGFGIVGFACTTCNGMSG 484
           +ARNANA GL RKPWVKTSLAPGSK V++YLEEANLL ELESLGFG+V FACTTCNGMSG
Sbjct: 421 IARNANALGLMRKPWVKTSLAPGSKTVKMYLEEANLLSELESLGFGVVAFACTTCNGMSG 480

Query: 485 ALDPVIQQEVIDRDLYATAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAIAGTIRFDIE 544
           ALDP IQQE++DRDLYATAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAIAGTIRFDIE
Sbjct: 481 ALDPAIQQEIVDRDLYATAVLSGNRNFDGRIHPYAKQAFLASPPLVVAYAIAGTIRFDIE 540

Query: 545 KDVLGLDKDGKPVRLINIWPSDAEIDAVIAASVKPEQFRKVYEPMFDLSVDYGDKVSPLY 604
           KD LG D  G PV L +IWPSD EIDA++  +VKPEQFR  Y PMFD        V PLY
Sbjct: 541 KDALGYDDKGNPVTLKDIWPSDEEIDAIVKRAVKPEQFRATYIPMFDADATSRTYVDPLY 600

Query: 605 DWRPQSTYIRRPPYWEGALAGERTLKGMRPLAVLGDNITTDHLSPSNAIMMDSAAGEYLH 664
           +WRPQSTYIRRPPYWEGALAGER+LKGMRPLAVL DNITTDHLSPSNAIM DSAAGEYL 
Sbjct: 601 EWRPQSTYIRRPPYWEGALAGERSLKGMRPLAVLPDNITTDHLSPSNAIMADSAAGEYLA 660

Query: 665 KMGLPEEDFNSYATHRGDHLTAQRATFANPKLKNEMAIVDGKVKQGSLARIEPEGIVTRM 724
           KMG+PEEDFNSYATHRGDHLTAQRATFANPKL NEMA+V+G+V  GSLARIEPEG V RM
Sbjct: 661 KMGVPEEDFNSYATHRGDHLTAQRATFANPKLINEMAVVNGEVVHGSLARIEPEGQVVRM 720

Query: 725 WEAIETYMDRKQPLIIIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLVGMG 784
           WEAIETYM RKQPLIIIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNL+GMG
Sbjct: 721 WEAIETYMQRKQPLIIIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMG 780

Query: 785 VLPLEFKAGENRATYGIDGTEVFDVIGSIAPRADLTVIITRKNGERVEVPVTCRLDTAEE 844
           VLPLEF +G  R T GIDGTE FDVIG  APRA + ++I   NGE +E PV CRLDT EE
Sbjct: 781 VLPLEFPSGTTRKTLGIDGTETFDVIGEFAPRATMRLVIRLANGEGIETPVICRLDTQEE 840

Query: 845 VSIYEAGGVLQRFAQDFLESN 865
           VS ++AGGVLQRFAQDFLE++
Sbjct: 841 VSTFQAGGVLQRFAQDFLEAS 861


Lambda     K      H
   0.318    0.136    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 2224
Number of extensions: 83
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 867
Length of database: 866
Length adjustment: 42
Effective length of query: 825
Effective length of database: 824
Effective search space:   679800
Effective search space used:   679800
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory