GapMind for catabolism of small carbon sources

 

Protein WP_033100348.1 in Thermoactinomyces daqus H-18

Annotation: NCBI__GCF_000763315.1:WP_033100348.1

Length: 262 amino acids

Source: GCF_000763315.1 in NCBI

Candidate for 26 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
4-hydroxybenzoate catabolism ech hi crotonase (EC 4.2.1.150) (characterized) 51% 100% 261.2 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) 48% 238.4
L-arginine catabolism ech hi crotonase (EC 4.2.1.150) (characterized) 51% 100% 261.2 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) 48% 238.4
L-citrulline catabolism ech hi crotonase (EC 4.2.1.150) (characterized) 51% 100% 261.2 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) 48% 238.4
L-lysine catabolism ech hi crotonase (EC 4.2.1.150) (characterized) 51% 100% 261.2 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) 48% 238.4
phenylacetate catabolism ech hi crotonase (EC 4.2.1.150) (characterized) 51% 100% 261.2 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) 48% 238.4
L-phenylalanine catabolism ech hi crotonase (EC 4.2.1.150) (characterized) 51% 100% 261.2 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) 48% 238.4
L-proline catabolism ech hi crotonase (EC 4.2.1.150) (characterized) 51% 100% 261.2 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) 48% 238.4
L-valine catabolism ech hi crotonase (EC 4.2.1.150) (characterized) 51% 100% 261.2 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) 48% 238.4
L-isoleucine catabolism hpcD med 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) (characterized) 48% 100% 238.4 crotonase (EC 4.2.1.150) 51% 261.2
propionate catabolism hpcD med 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) (characterized) 48% 100% 238.4 crotonase (EC 4.2.1.150) 51% 261.2
L-threonine catabolism hpcD med 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) (characterized) 48% 100% 238.4 crotonase (EC 4.2.1.150) 51% 261.2
L-valine catabolism hpcD med 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) (characterized) 48% 100% 238.4 crotonase (EC 4.2.1.150) 51% 261.2
4-hydroxybenzoate catabolism paaF med 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) (characterized) 49% 94% 222.2 crotonase (EC 4.2.1.150) 51% 261.2
L-isoleucine catabolism ech med 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) (characterized) 49% 94% 222.2 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) 48% 238.4
phenylacetate catabolism paaF med 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) (characterized) 49% 94% 222.2 crotonase (EC 4.2.1.150) 51% 261.2
L-phenylalanine catabolism paaF med 2,3-dehydroadipyl-CoA hydratase (EC 4.2.1.17) (characterized) 49% 94% 222.2 crotonase (EC 4.2.1.150) 51% 261.2
L-leucine catabolism liuC med methylglutaconyl-CoA hydratase (EC 4.2.1.18) (characterized) 44% 81% 192.2 crotonase (EC 4.2.1.150) 51% 261.2
4-hydroxybenzoate catabolism badK lo BadK (characterized) 38% 95% 166 crotonase (EC 4.2.1.150) 51% 261.2
phenylacetate catabolism badK lo BadK (characterized) 38% 95% 166 crotonase (EC 4.2.1.150) 51% 261.2
L-phenylalanine catabolism badK lo BadK (characterized) 38% 95% 166 crotonase (EC 4.2.1.150) 51% 261.2
phenylacetate catabolism paaG lo 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) (characterized) 38% 97% 159.8 crotonase (EC 4.2.1.150) 51% 261.2
L-phenylalanine catabolism paaG lo 2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA isomerase (EC 5.3.3.18) (characterized) 38% 97% 159.8 crotonase (EC 4.2.1.150) 51% 261.2
4-hydroxybenzoate catabolism dch lo cyclohexa-1,5-dienecarbonyl-CoA hydratase monomer (EC 4.2.1.100) (characterized) 32% 99% 130.6 crotonase (EC 4.2.1.150) 51% 261.2
phenylacetate catabolism dch lo cyclohexa-1,5-dienecarbonyl-CoA hydratase monomer (EC 4.2.1.100) (characterized) 32% 99% 130.6 crotonase (EC 4.2.1.150) 51% 261.2
L-phenylalanine catabolism dch lo cyclohexa-1,5-dienecarbonyl-CoA hydratase monomer (EC 4.2.1.100) (characterized) 32% 99% 130.6 crotonase (EC 4.2.1.150) 51% 261.2
L-valine catabolism bch lo 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial; 3-hydroxyisobutyryl-coenzyme A hydrolase; HIB-CoA hydrolase; HIBYL-CoA-H; EC 3.1.2.4 (characterized) 31% 53% 92.8 crotonase (EC 4.2.1.150) 51% 261.2

Sequence Analysis Tools

View WP_033100348.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MSDWKNVQVEKGDAWALVTFNRPKVLNALNRELLEELDAVISWIEQDEEIRVLIMTGSGE
KAFVAGADITELRRIPSATEAERLAAFGQRLFSRIESLPIPVIMAVNGYALGGGCELAMA
GDIILASDRARFGQPEINLGVIPGYGGTQRLARRVGTGTAKYLCMTGEMIDAQEAARLGL
VQKVVAADQLLDEAKRLAKQLAQKAPIALRYIKKAIDIGTETDLSAGLHLEASYFGLTFN
TDDRAEGMDAFLEKRPPRFTGK

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory