GapMind for catabolism of small carbon sources

 

Protein WP_033101601.1 in Thermoactinomyces daqus H-18

Annotation: NCBI__GCF_000763315.1:WP_033101601.1

Length: 422 amino acids

Source: GCF_000763315.1 in NCBI

Candidate for 7 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-asparagine catabolism glt hi Proton/sodium-glutamate symport protein; Glutamate-aspartate carrier protein (characterized) 75% 98% 624.4 The dicarboxylate (succinate, fumarate, malate and oxaloacetate):H+ symporter, DctA (probably 3H+ are transported per succinate taken up 44% 385.2
L-aspartate catabolism glt hi Proton/sodium-glutamate symport protein; Glutamate-aspartate carrier protein (characterized) 75% 98% 624.4 The dicarboxylate (succinate, fumarate, malate and oxaloacetate):H+ symporter, DctA (probably 3H+ are transported per succinate taken up 44% 385.2
L-glutamate catabolism gltP hi Proton/sodium-glutamate symport protein; Glutamate-aspartate carrier protein (characterized) 75% 98% 624.4 The dicarboxylate (succinate, fumarate, malate and oxaloacetate):H+ symporter, DctA (probably 3H+ are transported per succinate taken up 44% 385.2
L-malate catabolism dctA med The dicarboxylate (succinate, fumarate, malate and oxaloacetate):H+ symporter, DctA (probably 3H+ are transported per succinate taken up (characterized) 44% 97% 385.2 Proton/sodium-glutamate symport protein; Glutamate-aspartate carrier protein 75% 624.4
fumarate catabolism dctA med The dicarboxylate (succinate, fumarate, malate and oxaloacetate):H+ symporter, DctA (probably 3H+ are transported per succinate taken up (characterized) 44% 97% 385.2 Proton/sodium-glutamate symport protein; Glutamate-aspartate carrier protein 75% 624.4
succinate catabolism dctA med The dicarboxylate (succinate, fumarate, malate and oxaloacetate):H+ symporter, DctA (probably 3H+ are transported per succinate taken up (characterized) 44% 97% 385.2 Proton/sodium-glutamate symport protein; Glutamate-aspartate carrier protein 75% 624.4
acetate catabolism dctA med Organic acid uptake porter, DctA of 444 aas and 8 - 10 putative TMSs (characterized) 43% 93% 352.4 Proton/sodium-glutamate symport protein; Glutamate-aspartate carrier protein 75% 624.4

Sequence Analysis Tools

View WP_033101601.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MKRISLAWQILIGLVLGIAVGAVFYGNPAVETYLQPVGDIFLHLIKMIVVPIVFSTLVVG
VAGVGDVKKLGKLGGKTILYFEIITTIAIIVGLLVANIVQPGDGVNMKQLTKTDIQSYVD
TANQQNTHSFVDTVVGIVPTNPFAAFANGDMLAVIFFSVMFGLAVAAIGERGKPVLAFFK
GASDAMFWVTNKVMRFAPFGVFALIGVTISKFGIHSLVPLLKLVLSVYGAMILFVVLILG
LVAKFVCKVNLWTMIKILKDELILAYSTASSETVLPRIMEKMEKFGCPKSITSFVIPTGY
SFNLDGSTLYQALAALFIAQMYNIHMPIEKQIMLVLVLMLTSKGIAGVPGVSFVVLLATL
GSVGIPLEGLAFIAGIDRILDMGRTAVNVVGNSLAAVVISTWEKQYDREKGNAYARGEGE
AA

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory