Alignments for a candidate for pgmA in Thermoactinomyces daqus H-18

GapMind for catabolism of small carbon sources

Alignments for a candidate for pgmA in Thermoactinomyces daqus H-18

Align Phosphoglucomutase; PGM; Alpha-phosphoglucomutase; Glucose phosphomutase; EC 5.4.2.2 (characterized)
to candidate WP_033101249.1 JG50_RS0111465 phospho-sugar mutase

Query= SwissProt::P18159
         (581 letters)



>NCBI__GCF_000763315.1:WP_033101249.1
          Length = 565

 Score =  495 bits (1274), Expect = e-144
 Identities = 255/574 (44%), Positives = 371/574 (64%), Gaps = 20/574 (3%)

Query: 1   MTWRKSYERWKQTEHLDLELKERLIELEGDEQALEDCFYKDLEFGTGGMRGEIGAGTNRM 60
           M +R +++ W      D + K  L  L  DE+ +ED FY+DL+FGTGG+RG IGAGTNR+
Sbjct: 1   MNYRDAFDYWMTDSFFDEKTKLELSMLT-DEKEIEDRFYQDLKFGTGGVRGLIGAGTNRI 59

Query: 61  NIYTVRKASAGFAAYISKQ-GEEAKKRGVVIAYDSRHKSPEFAMEAAKTLATQGIQTYVF 119
           N+YT+RK + G A Y+  + GE+AK++GV IA+DSR  S  FA EAA  L   GI+ ++F
Sbjct: 60  NLYTIRKITCGLANYLQAEFGEQAKRQGVAIAFDSRLNSSAFAKEAALVLCATGIKVFLF 119

Query: 120 DELRPTPELSFAVRQLNAYGGIVVTASHNPPEYNGYKVYGDDGGQLPPKEADIVIEQVNA 179
           D L PTP LSF V+ L    GIV+TASHNP +YNGYKVY + G QL P+  D +   V+A
Sbjct: 120 DRLMPTPVLSFTVKHLGCIAGIVITASHNPKDYNGYKVYDNKGCQLVPRYTDRITRYVDA 179

Query: 180 IENELTITVDEENKLKEKGLIKIIGEDIDKVYTEKLTSISVHPELSEEVDVKVVFTPLHG 239
           + +   I+V    +   +G++KIIG ++   + +++   +   +   +  +K+V+TPLHG
Sbjct: 180 VTDLTKISVANYEQAMAEGMLKIIGTEVLDAFIDEVLMQTCFDDADAKAALKIVYTPLHG 239

Query: 240 TANKPVRRGLEALGYKNVTVVKEQELPDSNFSTVTSPNPEEHAAFEYAIKLGEEQNADIL 299
           T N PVR  LE  G+  V+VVK QE PDS+FSTV+SPNPE+ +A E  I+       DI+
Sbjct: 240 TGNLPVRSVLERDGFTGVSVVKAQEFPDSSFSTVSSPNPEDRSALELGIEQAIAGGDDIV 299

Query: 300 IATDPDADRLGIAVKNDQGKYTVLTGNQTGALLLHYLLSEKKKQGILPDNGVVLKTIVTS 359
           + TDPD DR+G+AV++ +G +T+LTGNQ GALL+H++LS++K    L +   ++KTIVT 
Sbjct: 300 LGTDPDCDRVGVAVRSGEG-FTLLTGNQIGALLVHFVLSQRKH--ALNEKSTLVKTIVTD 356

Query: 360 EIGRAVASSFGLDTIDTLTGFKFIGEKIKEYEASGQYTFQFGYEESYGYLIGDFARDKDA 419
           E+G  +A S+GL+ +DTLTGFK+IG++I  YE +G+  F  GYEESYG+L+G  ARDKDA
Sbjct: 357 ELGAKIAQSYGLNIVDTLTGFKYIGDQITLYEQTGEKEFFIGYEESYGFLVGMHARDKDA 416

Query: 420 IQAALLAVEVCAFYKKQGMSLYEALINLFNEYGFYREGLKSLTLKGKQGAEQIEAILASF 479
           I A++L  E+ A+YK QG +L   L +++  YG+Y   + S    GK G E++++++ + 
Sbjct: 417 IVASMLICEMAAYYKTQGKTLLNVLSDIYEHYGYYFNAIDSFEFNGKVGVEEMQSMMTTL 476

Query: 480 RQNPPQKMAGKQVVTAEDYAVSKRTLLTESKEEAID-LPKSNVLKYFLEDGSWFCLRPSG 538
           R      + G  ++   DY+              ID LPKSNVLKY L DGSW  +RPSG
Sbjct: 477 RNKGTTLITG--IIEIRDYST------------GIDGLPKSNVLKYILNDGSWIAIRPSG 522

Query: 539 TEPKVKFYFAVKGSSLEDSEKRLAVLSEDVMKTV 572
           TEPK+K Y++V       ++KRL ++   + K +
Sbjct: 523 TEPKIKVYYSVYQPDAYSAQKRLEMIQTTIRKEI 556


Lambda     K      H
   0.313    0.133    0.371 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 790
Number of extensions: 38
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 581
Length of database: 565
Length adjustment: 36
Effective length of query: 545
Effective length of database: 529
Effective search space:   288305
Effective search space used:   288305
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources