Alignments for a candidate for prpD in Microbacterium profundi Shh49

GapMind for catabolism of small carbon sources

Alignments for a candidate for prpD in Microbacterium profundi Shh49

Align 2-methylcitrate dehydratase 2; 2-MC dehydratase; Aconitate hydratase; ACN; Aconitase; EC 4.2.1.79; EC 4.2.1.3 (characterized)
to candidate WP_033105871.1 JF52_RS0109075 MmgE/PrpD family protein

Query= SwissProt::Q8NSL3
         (504 letters)



>NCBI__GCF_000763375.1:WP_033105871.1
          Length = 514

 Score =  668 bits (1723), Expect = 0.0
 Identities = 340/513 (66%), Positives = 402/513 (78%), Gaps = 11/513 (2%)

Query: 2   INHEVRTHRSAEEFPYEEHLAHKIARVAADPVEVAADTQEMIINRIIDNASVQAASVLRR 61
           + H VR ++S E+ P E  LA KIA VA D VEV  D  +MIINRIIDNASV AAS+ R 
Sbjct: 3   VTHHVRVYKSEEDLPRENQLAWKIAEVATDAVEVEQDVVDMIINRIIDNASVAAASLTRG 62

Query: 62  PVSSARAMAQVRPV-TDGRGASVFGLP--GRYAAEWAALANGTAVRELDFHDTFLAAEYS 118
           P+ +AR  A   PV T G+GA+VFG P     + EWAA ANG AVRELD+HDTFLAAEYS
Sbjct: 63  PIIAARDQAFSHPVSTGGKGATVFGAPLDAHTSPEWAAWANGVAVRELDYHDTFLAAEYS 122

Query: 119 HPGDNIPPILAAAQQAGKGGKDLIRGIATGYEIQVNLVRGMCLHEHKIDHVAHLGPSAAA 178
           HPGDNIPPILA AQ  G  G+ L+RGIATGYEIQV+LV+ +CLH+HKIDHVAHLGPSAAA
Sbjct: 123 HPGDNIPPILAVAQHTGADGRALVRGIATGYEIQVDLVKAICLHKHKIDHVAHLGPSAAA 182

Query: 179 GIGTLLDLDVDTIYQAIGQALHTTTATRQSRKGAISSWKAFAPAFAGKMSIEAVDRAMRG 238
           GIGTLL LD +TIYQA+ QALHTTTATRQSRKG IS+WKA APAFAGKM++EAVDRAMRG
Sbjct: 183 GIGTLLGLDAETIYQAVSQALHTTTATRQSRKGEISTWKAHAPAFAGKMAVEAVDRAMRG 242

Query: 239 EGAPSPIWEGEDGVIAWLLSGLDHIYTIPLPAEGEAKRAILDTYTKEHSAEYQSQAPIDL 298
           + +PSPI+EGEDGVIAW+L G D  Y +PLP +GEAKRAILD+YTKEHSAEYQ+QA IDL
Sbjct: 243 QTSPSPIYEGEDGVIAWMLDGPDAAYEVPLPPKGEAKRAILDSYTKEHSAEYQAQAWIDL 302

Query: 299 ARSMGEKL-----AAQGLDLRDVDSIVLHTSHHTHYVIGTGSNDPQKFDPDASRETLDHS 353
           AR + ++        +G    D++S+VLHTSHHTHYVIG+G+NDPQK+DP ASRETLDHS
Sbjct: 303 ARKLHKEYFSLPEPVEGRTWHDIESVVLHTSHHTHYVIGSGANDPQKYDPTASRETLDHS 362

Query: 354 IMYIFAVALEDRAWHHERSYAPERAHRRETIELWNKISTVEDPEWTRRYHSVDPAEKAFG 413
           I YIF VAL+D +WHH  SYAPERA R +T+ELWN+++T+ED EWTRRYHS D AEKAFG
Sbjct: 363 IPYIFTVALQDGSWHHVDSYAPERAGRADTVELWNRVTTLEDAEWTRRYHSNDIAEKAFG 422

Query: 414 ARAVITFKDGTVVEDELAVADAHPLGARPFAREQYIQKFRTLAEGVVSEKEQDRFLDAAQ 473
            R  ITF DG+ + DE+AVADAHPLGARPFARE YI KFR LAE V+S +E +RFL   Q
Sbjct: 423 GRVEITFTDGSTLVDEIAVADAHPLGARPFARENYIAKFRLLAEPVLSSEEIERFLSLVQ 482

Query: 474 RTHEL--EDLSELNIELDADILAKAPVIPEGLF 504
           R  EL   +++EL+I     ++A  P   +GLF
Sbjct: 483 RLPELTAAEVAELSIIAKPGVVASEPT-TKGLF 514


Lambda     K      H
   0.317    0.132    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 853
Number of extensions: 38
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 504
Length of database: 514
Length adjustment: 34
Effective length of query: 470
Effective length of database: 480
Effective search space:   225600
Effective search space used:   225600
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources