Protein WP_036137991.1 in Lysobacter daejeonensis GH1-9
Annotation: NCBI__GCF_000768355.1:WP_036137991.1
Length: 447 amino acids
Source: GCF_000768355.1 in NCBI
Candidate for 19 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| L-histidine catabolism | permease | hi | Aromatic amino acid permease, AroP (characterized) | 48% | 94% | 387.5 | GABA permease; 4-amino butyrate transport carrier; Gamma-aminobutyrate permease; Proline transporter GabP | 43% | 364.4 |
| L-phenylalanine catabolism | aroP | hi | Aromatic amino acid permease, AroP (characterized) | 48% | 94% | 387.5 | GABA permease; 4-amino butyrate transport carrier; Gamma-aminobutyrate permease; Proline transporter GabP | 43% | 364.4 |
| L-tryptophan catabolism | aroP | hi | Aromatic amino acid permease, AroP (characterized) | 48% | 94% | 387.5 | GABA permease; 4-amino butyrate transport carrier; Gamma-aminobutyrate permease; Proline transporter GabP | 43% | 364.4 |
| L-tyrosine catabolism | aroP | hi | Aromatic amino acid permease, AroP (characterized) | 48% | 94% | 387.5 | GABA permease; 4-amino butyrate transport carrier; Gamma-aminobutyrate permease; Proline transporter GabP | 43% | 364.4 |
| L-proline catabolism | proY | med | GABA permease; 4-amino butyrate transport carrier; Gamma-aminobutyrate permease; Proline transporter GabP (characterized) | 43% | 91% | 364.4 | Aromatic amino acid permease, AroP | 48% | 387.5 |
| D-alanine catabolism | cycA | lo | L-alanine and D-alanine permease (characterized) | 36% | 93% | 290 | Aromatic amino acid permease, AroP | 48% | 387.5 |
| L-alanine catabolism | cycA | lo | L-alanine and D-alanine permease (characterized) | 36% | 93% | 290 | Aromatic amino acid permease, AroP | 48% | 387.5 |
| L-threonine catabolism | RR42_RS28305 | lo | D-serine/D-alanine/glycine transporter (characterized, see rationale) | 38% | 90% | 288.1 | Aromatic amino acid permease, AroP | 48% | 387.5 |
| phenylacetate catabolism | H281DRAFT_04042 | lo | Aromatic amino acid transporter AroP (characterized, see rationale) | 36% | 94% | 269.6 | Aromatic amino acid permease, AroP | 48% | 387.5 |
| L-arginine catabolism | rocE | lo | Amino-acid permease RocE (characterized) | 33% | 93% | 251.9 | Aromatic amino acid permease, AroP | 48% | 387.5 |
| L-lysine catabolism | lysP | lo | lysine-specific permease (characterized) | 36% | 85% | 251.1 | Aromatic amino acid permease, AroP | 48% | 387.5 |
| D-serine catabolism | cycA | lo | D-serine/L-alanine/D-alanine/glycine/D-cycloserine uptake porter of 556 aas, CycA (characterized) | 36% | 79% | 245.7 | Aromatic amino acid permease, AroP | 48% | 387.5 |
| L-asparagine catabolism | ansP | lo | Asparagine permease (AnsP) of 497 aas and 12 TMSs (characterized) | 33% | 88% | 239.6 | Aromatic amino acid permease, AroP | 48% | 387.5 |
| L-isoleucine catabolism | Bap2 | lo | Arbuscular mycorrhizal fungal proline:H+ symporter, AAP1 (binds and probably transports nonpolar, hydrophobic amino acids) (characterized) | 35% | 80% | 218.4 | Aromatic amino acid permease, AroP | 48% | 387.5 |
| L-leucine catabolism | Bap2 | lo | Arbuscular mycorrhizal fungal proline:H+ symporter, AAP1 (binds and probably transports nonpolar, hydrophobic amino acids) (characterized) | 35% | 80% | 218.4 | Aromatic amino acid permease, AroP | 48% | 387.5 |
| L-valine catabolism | Bap2 | lo | Arbuscular mycorrhizal fungal proline:H+ symporter, AAP1 (binds and probably transports nonpolar, hydrophobic amino acids) (characterized) | 35% | 80% | 218.4 | Aromatic amino acid permease, AroP | 48% | 387.5 |
| L-serine catabolism | serP | lo | Serine uptake transporter, SerP1, of 259 aas and 12 TMSs (Trip et al. 2013). L-serine is the highest affinity substrate (Km = 18 μM), but SerP1 also transports L-threonine and L-cysteine (Km values = 20 - 40 μM) (characterized) | 31% | 95% | 202.6 | Aromatic amino acid permease, AroP | 48% | 387.5 |
| L-threonine catabolism | serP1 | lo | Serine uptake transporter, SerP1, of 259 aas and 12 TMSs (Trip et al. 2013). L-serine is the highest affinity substrate (Km = 18 μM), but SerP1 also transports L-threonine and L-cysteine (Km values = 20 - 40 μM) (characterized) | 31% | 95% | 202.6 | Aromatic amino acid permease, AroP | 48% | 387.5 |
| L-tryptophan catabolism | TAT | lo | tryptophan permease (characterized) | 31% | 71% | 198.7 | Aromatic amino acid permease, AroP | 48% | 387.5 |
Sequence Analysis Tools
View WP_036137991.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
Sequence
MAAADTTPLQHALKPRQLIMMGLGTAIGAGLFLGSGVGIQAAGPAVLVSYLIAGALVIIV
MHALGEMAAAHPASGAFSVYAADAFGPVAGATVGWLWWLQVVIVVAAESVGAAGLLATLW
PALPVPWLSLGFMVVFTAINLLGVRNFGEFEFWFAIVKVVAILAFIAIGIALLMGWLPQV
TPPGWSNFTAHGGFAPKGLAGIGAALLVVVFAFGGTEIVAIAAAESEHPGRSLAQAIRTV
AWRILVFYLGSLAVIIAVVPWTSEALKSPFAAVLEVARIPGAAAAITLVAVVALLSALNA
NLYGASRMIYSLAQRNEAPRALARLDAQRVPVLAVLASVAFGFFATALELRWPDKVLPAL
LNLVGSTCLMVWTITLASQWVLRRRADRQGRGLPFRMRGFPYVTLAGLGILGLIFALTLL
SPASRTQLLSTVALTVAIAGLCRWAKR
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Links
Downloads
Related tools
About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory