Alignments for a candidate for aacS in Lysobacter daejeonensis GH1-9

GapMind for catabolism of small carbon sources

Alignments for a candidate for aacS in Lysobacter daejeonensis GH1-9

Align Acetoacetate--CoA ligase (EC 6.2.1.16) (characterized)
to candidate WP_156962857.1 N800_RS03285 AMP-binding protein

Query= reanno::acidovorax_3H11:Ac3H11_3009
         (578 letters)



>NCBI__GCF_000768355.1:WP_156962857.1
          Length = 504

 Score =  191 bits (486), Expect = 5e-53
 Identities = 145/537 (27%), Positives = 239/537 (44%), Gaps = 44/537 (8%)

Query: 29  QTIGAFFADMVARQPEREALVSVHQGRRYTYAQLQTEAHRLASALLGMGLTPGDRVGIWS 88
           Q++ +  A      P++ A+V     R+ +Y      A   A  L   GL  GDRV I  
Sbjct: 2   QSLASTVAMWAHSTPDKPAIVDAT--RQVSYRDFMAHAAGFAGQLARHGLRHGDRVAIVL 59

Query: 89  HNNAEWVLMQLATAQVGLVLVNINPAYRTAEVEYALNKVGCKLLVSMARFKTSDYLGMLR 148
            N  E  +    T   G V V +N   R  ++   L   G + +V       +D    + 
Sbjct: 60  PNRIEAAVACYGTWLAGGVAVPLNAQARARDIAPWLVHCGARFIVHEPG--NNDVASAME 117

Query: 149 ELAPEWQGQQPGHLQAAKLPQLKTVVWIDDEAGQGADEPGLLRFTELIARGNAADPRLAQ 208
            LA                P     V  D      +  PG      L AR    D  LA 
Sbjct: 118 TLAS---------------PPFPFTVPEDQPICPPSPLPG---DALLSARAPGPDGDLAL 159

Query: 209 VAAGLQATDPINIQFTSGTTGFPKGATLTHRNILNNGFFIGECMKLTPADRLCIPVPLYH 268
           +             +TSGTTG PKG  L+H N++ N   +   + L P+D +   +P Y+
Sbjct: 160 IL------------YTSGTTGAPKGVMLSHANLMANATAVAAYLALDPSDSVLCVLPFYY 207

Query: 269 CFGMVLGNLACFTHGATIVYPNDGFDPLTVLQTVQDERCTGLHGVPTMFIAELDHPRFAE 328
            +G  + +    + GAT+        P  +++ +   R TG  GVP+ F   L H     
Sbjct: 208 AYGASVVHTHLIS-GATLHLAGSLVFPNLIVEALATRRITGFSGVPSTFALLLSHGALDR 266

Query: 329 FNLSTLRTGIMAGSPCPTEVMKRVVEQMNLREITIAYGMTETSPVSCQSSTDTP---LSK 385
            +LSTLR    AG P    +  ++   +   ++ + YG TE +       T  P   L  
Sbjct: 267 HDLSTLRYVTQAGGPMSPSLTAQLRAALPGPQLFVMYGQTEAT----SRLTFLPPGQLDA 322

Query: 386 RVSTVGQVQPHLEVKIVDPDTGAVVPIGQRGEFCTKGYSVMHGYWGDEAKTREAIDEGGW 445
           +  +VG     + ++IV  + G     G+ G+   +G +VM GYW +   TR  + +G W
Sbjct: 323 KAGSVGIPVDGVSIRIVREE-GVPAAAGETGDVWVRGPNVMLGYWRNAEATRATLKDG-W 380

Query: 446 MHTGDLATMDAEGYVNIVGRIKDMVIRGGENIYPREIEEFLYRHPQVQDVQVVGVPDQKY 505
           + TGD+  +D +GY+ +VGR  DM+  G   ++P ++E+ +     V +V VVGV D   
Sbjct: 381 LKTGDMGHLDDDGYLYLVGRRSDMIKTGAHRVHPVDVEQAIAEMAGVIEVAVVGVDDDVL 440

Query: 506 GEELCAWIIAKPGTQPTEDDIRAFCKGQIAHYKVPRYIRFVTSFPMTVTGKIQKFKI 562
           G+ L A+++ +  T    D+I+A C+  +  YK+P+++ FV++ P T +GK+Q+ ++
Sbjct: 441 GQVLKAFVVVERVTTRLTDEIKAHCRAHLPPYKIPKHVEFVSTLPRTASGKVQRTQL 497


Lambda     K      H
   0.320    0.136    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 683
Number of extensions: 23
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 578
Length of database: 504
Length adjustment: 35
Effective length of query: 543
Effective length of database: 469
Effective search space:   254667
Effective search space used:   254667
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources