Align NatE, component of The neutral amino acid permease, N-1 (transports pro, phe, leu, gly, ala, ser, gln and his, but gln and his are not transported via NatB) (characterized)
to candidate WP_036134248.1 N800_RS01150 ATP-binding cassette domain-containing protein
Query= TCDB::Q8YT15 (247 letters) >NCBI__GCF_000768355.1:WP_036134248.1 Length = 309 Score = 105 bits (261), Expect = 1e-27 Identities = 66/206 (32%), Positives = 112/206 (54%), Gaps = 9/206 (4%) Query: 24 DVDILQGVNFRVESGELVTVIGPNGAGKSTLAKTIFGLLTPHTGKITFKGKNIAGLKSNQ 83 D+ + V+ V G + +GPNG+GKST + + GLLTP G + G +I + + Sbjct: 12 DLVAVDAVDLDVPRGHVYGFLGPNGSGKSTTIRMLCGLLTPSAGNVRVLGLDIP--REAE 69 Query: 84 IVRLGMCYVPQIANVFPSLSVEENLEMGAFIRNDSLQPLKDKIFAMFPR--LSDRRRQRA 141 +RL + Y+ Q ++F L+V ENLE A ++ S + +I + + DR RQ A Sbjct: 70 ALRLRIGYMTQRFSLFEDLTVHENLEFMATVQGLSRHDARRRIDELLQQYHFQDRSRQLA 129 Query: 142 GTLSGGERQMLAMGKALMLEPSLLVLDEPSAALSPILVTQVFEQVKQINQEGTAIILVEQ 201 GT+SGG++Q LA+ +++ P LL LDEP++A+ P +E++ ++ GT +++ Sbjct: 130 GTMSGGQKQRLALACSVVHRPELLFLDEPTSAVDPESRRDFWEKLFELADAGTTLLVSTH 189 Query: 202 NARKA-----LEMADRGYVLESGRDA 222 +A L + DRG ++ G A Sbjct: 190 YMDEAERCHRLAILDRGVLVADGTPA 215 Lambda K H 0.317 0.136 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 182 Number of extensions: 11 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 247 Length of database: 309 Length adjustment: 25 Effective length of query: 222 Effective length of database: 284 Effective search space: 63048 Effective search space used: 63048 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory