Annotation: NCBI__GCF_000768675.1:WP_035949051.1
Length: 551 amino acids
Source: GCF_000768675.1 in NCBI
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|---|---|---|---|---|---|---|---|---|
| acetate catabolism | actP | hi | Monocarboxylic acid transporter (characterized) | 60% | 100% | 633.6 | Phenylacetate permease, Ppa | 44% | 422.2 |
| propionate catabolism | mctC | hi | Monocarboxylic acid transporter (characterized) | 60% | 100% | 633.6 | Acetate permease ActP-2/ActP2/ActP3 | 45% | 431.4 |
| pyruvate catabolism | mctC | hi | Monocarboxylic acid transporter (characterized) | 60% | 100% | 633.6 | Acetate permease ActP-2/ActP2/ActP3 | 45% | 431.4 |
| pyruvate catabolism | actP | med | Acetate transporter (characterized, see rationale) | 44% | 97% | 440.7 | Monocarboxylic acid transporter | 60% | 633.6 |
| phenylacetate catabolism | ppa | med | Phenylacetate permease, Ppa (characterized) | 44% | 99% | 422.2 | Monocarboxylic acid transporter | 60% | 633.6 |
| L-lactate catabolism | Shew_2731 | lo | actP-like component of L-lactate and L-malate uptake system (characterized) | 35% | 56% | 156.4 | Monocarboxylic acid transporter | 60% | 633.6 |
View WP_035949051.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
MTSFLSAGLPAEATKVGSPALNIAIFAGFVLVTLVVIVKLASGKKSAGEFYTASGGFSGR QNGIAIAGDYLSAASFLGIAGAIALQGYDGFLYSIGFLVAWLVALLLVAELMRNTGRLTM ADVLSYRLKQRPVRIATATSVLAVSFFYLLAQMAGAGGLVSLLLGVEGEGAQNIVIAVVG IVMIAYVLIGGMRGTTWVQIIKAVLLIAATAIMTVWVLGKYGFNLSGLFDAAIQNNPKVG EKLLEPGVKYGVSTTTKIDFISLSLALVLGTAGLPHVLQRFYTVPTSKQARKSVEWAIWL IGGFYLLTLVVGYGAGALVGAEEINKAPGKANAAAPLLAFELGGSPFLGIVSGIAFATIL AVVAGLTITASATFAHDIYKEVIKRGDTTSDNEVRVARISAVAIGIIAILGGMFAKSQNI AFLVALAFAVAASANLPTILYSLFWKRFNTRGALWSIYGGLISTIGLIIFSPVVSGKAPL PGTTVSPSMITNPDIDFSWFPLDNPGLVTIPLAFFLGWLGTVTSSEYNAQKYAEMEVRSL TGHGVAKALDH
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory