GapMind for catabolism of small carbon sources

 

Protein WP_035130836.1 in Flavobacterium beibuense F44-8

Annotation: NCBI__GCF_000769915.1:WP_035130836.1

Length: 414 amino acids

Source: GCF_000769915.1 in NCBI

Candidate for 17 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-arginine catabolism rocD hi Ornithine aminotransferase (EC 2.6.1.13) (characterized) 71% 99% 610.9 ornithine aminotransferase 57% 467.6
L-arginine catabolism rocD med rocD: ornithine--oxo-acid transaminase (EC 2.6.1.13) (TIGR01885) 100% 678.2 ornithine aminotransferase 57% 467.6
L-citrulline catabolism rocD hi Ornithine aminotransferase (EC 2.6.1.13) (characterized) 71% 99% 610.9 ornithine aminotransferase 57% 467.6
L-citrulline catabolism rocD med rocD: ornithine--oxo-acid transaminase (EC 2.6.1.13) (TIGR01885) 100% 678.2 ornithine aminotransferase 57% 467.6
L-arginine catabolism astC lo succinylornithine transaminase (EC 2.6.1.81) (characterized) 34% 95% 240.7 Ornithine aminotransferase (EC 2.6.1.13) 71% 610.9
L-citrulline catabolism astC lo succinylornithine transaminase (EC 2.6.1.81) (characterized) 34% 95% 240.7 Ornithine aminotransferase (EC 2.6.1.13) 71% 610.9
L-arginine catabolism davT lo 5-aminovalerate transaminase (EC 2.6.1.48) (characterized) 33% 96% 216.1 Ornithine aminotransferase (EC 2.6.1.13) 71% 610.9
L-citrulline catabolism davT lo 5-aminovalerate transaminase (EC 2.6.1.48) (characterized) 33% 96% 216.1 Ornithine aminotransferase (EC 2.6.1.13) 71% 610.9
L-lysine catabolism davT lo 5-aminovalerate transaminase (EC 2.6.1.48) (characterized) 33% 96% 216.1 Ornithine aminotransferase (EC 2.6.1.13) 71% 610.9
L-proline catabolism davT lo 5-aminovalerate transaminase (EC 2.6.1.48) (characterized) 33% 96% 216.1 Ornithine aminotransferase (EC 2.6.1.13) 71% 610.9
L-arginine catabolism gabT lo 4-aminobutyrate aminotransferase; EC 2.6.1.19 (characterized, see rationale) 32% 92% 213.4 Ornithine aminotransferase (EC 2.6.1.13) 71% 610.9
L-citrulline catabolism gabT lo 4-aminobutyrate aminotransferase; EC 2.6.1.19 (characterized, see rationale) 32% 92% 213.4 Ornithine aminotransferase (EC 2.6.1.13) 71% 610.9
putrescine catabolism gabT lo 4-aminobutyrate aminotransferase; EC 2.6.1.19 (characterized, see rationale) 32% 92% 213.4 Ornithine aminotransferase (EC 2.6.1.13) 71% 610.9
L-arginine catabolism patA lo putrescine-2-oxoglutarate transaminase (EC 2.6.1.82) (characterized) 35% 81% 207.6 Ornithine aminotransferase (EC 2.6.1.13) 71% 610.9
L-citrulline catabolism patA lo putrescine-2-oxoglutarate transaminase (EC 2.6.1.82) (characterized) 35% 81% 207.6 Ornithine aminotransferase (EC 2.6.1.13) 71% 610.9
L-lysine catabolism patA lo putrescine-2-oxoglutarate transaminase (EC 2.6.1.82) (characterized) 35% 81% 207.6 Ornithine aminotransferase (EC 2.6.1.13) 71% 610.9
putrescine catabolism patA lo putrescine-2-oxoglutarate transaminase (EC 2.6.1.82) (characterized) 35% 81% 207.6 Ornithine aminotransferase (EC 2.6.1.13) 71% 610.9
L-lysine catabolism lat lo Ornithine aminotransferase; Orn-AT; Lysine aminotransferase; Lys-AT; EC 2.6.1.13; EC 2.6.1.36 (characterized) 32% 91% 204.1 Ornithine aminotransferase (EC 2.6.1.13) 71% 610.9
L-lysine catabolism lysN lo 2-aminoadipate transaminase (EC 2.6.1.39) (characterized) 30% 97% 172.2 Ornithine aminotransferase (EC 2.6.1.13) 71% 610.9

Sequence Analysis Tools

View WP_035130836.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MSVLEKLSSADAIALEDKYGAHNYHPLPVVLSKGEGVYVWDVEGKKYYDFLSAYSAVNQG
HCHPKIVGAMMEQAQTLTLTSRAFYNDKLGRYEEFITKYFGFDKVLPMNTGAEAVETAIK
LCRKWAYEKKGIAEQQAKIVVCKNNFHGRTTTIISFSNDEDARKNFGPYTEGFIKIEYDN
LEVLEETLKNNSDIAGFLVEPIQGEAGVYVPSEGYLAKAKALCEKHNVLFIADEVQTGIA
RTGKLLAVNHENVTPDILILGKALSGGAYPVSAVLANNSVMDVIKPGQHGSTFGGNPVAA
AVAIAALEVIQDEKLAENAEKLGVLFRDKINEYISESNIVTLVRGKGLLNAIVINDTEDS
STAWDICVKLKEHGLLAKPTHGNIIRFAPPLVMNEEQLLDCVNIITSTLKEFEK

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory