GapMind for catabolism of small carbon sources

 

Alignments for a candidate for mtlK in Flavobacterium beibuense F44-8

Align MtlK, component of The polyol (mannitol, glucitol (sorbitol), arabitol (arabinitol; lyxitol)) uptake porter, MtlEFGK (characterized)
to candidate WP_035136158.1 Q763_RS16555 ABC transporter ATP-binding protein

Query= TCDB::O30494
         (367 letters)



>NCBI__GCF_000769915.1:WP_035136158.1
          Length = 234

 Score =  123 bits (308), Expect = 6e-33
 Identities = 79/219 (36%), Positives = 118/219 (53%), Gaps = 12/219 (5%)

Query: 4   LKIKNLQKGFEGFSI----IKGIDLEVNDKEFVVFVGPSGCGKSTLLRLIAGLEEVSEGT 59
           +KI+NL K F    +    +  I +EV   EFV  +G SGCGKSTLL ++  L+  S G+
Sbjct: 2   IKIENLSKVFRTEEVETKALNDISIEVKKGEFVTIMGASGCGKSTLLNIVGLLDSASGGS 61

Query: 60  IELDGRDITEVTPA------KRDLAMVFQTYALYPHMSVRKNMSFALDLAGVDKQLVESK 113
            +L  R+I  ++ +      K ++  VFQ + L   +SV  N+   L    V     + +
Sbjct: 62  YKLLDREINGLSESEKAKVRKENIGFVFQNFNLIDELSVYDNIELPLIYNNVPSGERKKR 121

Query: 114 VNEAARILELGPLLERKPKQLSGGQRQRVAIGRAIVRNPKIFLFDEPLSNLDAALRVQMR 173
           V E A  L +   L+  P+QLSGGQ+QR A+ RA+V NPKI L DEP  NLD+    ++ 
Sbjct: 122 VEEIAERLGISHRLKHYPQQLSGGQQQRAAVARALVNNPKIILADEPTGNLDSKNGNEVM 181

Query: 174 LELARLHKELQATMIYVTHDQVEAMTLADKVVVLNSGRI 212
             L  LH    AT++ VTH + +A + + K + +  G I
Sbjct: 182 ELLTDLHAN-GATILMVTHSEYDA-SFSQKTIYMKDGMI 218


Lambda     K      H
   0.319    0.137    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 154
Number of extensions: 8
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 367
Length of database: 234
Length adjustment: 26
Effective length of query: 341
Effective length of database: 208
Effective search space:    70928
Effective search space used:    70928
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory