GapMind for catabolism of small carbon sources

 

Alignments for a candidate for malK in Flavobacterium beibuense F44-8

Align MsmK aka SMU.882, component of The raffinose/stachyose transporter, MsmEFGK (MalK (3.A.1.1.27) can probably substitute for MsmK; Webb et al., 2008). This system may also transport melibiose, isomaltotriose and sucrose as well as isomaltosaccharides (characterized)
to candidate WP_035136158.1 Q763_RS16555 ABC transporter ATP-binding protein

Query= TCDB::Q00752
         (377 letters)



>NCBI__GCF_000769915.1:WP_035136158.1
          Length = 234

 Score =  120 bits (302), Expect = 3e-32
 Identities = 67/186 (36%), Positives = 104/186 (55%), Gaps = 7/186 (3%)

Query: 20  SVEDFDLDIKNKEFIVFVGPSGCGKSTTLRMVAGLEDITKGELKIDGEVVNDKAPKDR-- 77
           ++ D  +++K  EF+  +G SGCGKST L +V  L+  + G  K+    +N  +  ++  
Sbjct: 20  ALNDISIEVKKGEFVTIMGASGCGKSTLLNIVGLLDSASGGSYKLLDREINGLSESEKAK 79

Query: 78  ----DIAMVFQNYALYPHMSVYDNMAFGLKLRHYSKEAIDKRVKEAAQILGLTEFLERKP 133
               +I  VFQN+ L   +SVYDN+   L   +       KRV+E A+ LG++  L+  P
Sbjct: 80  VRKENIGFVFQNFNLIDELSVYDNIELPLIYNNVPSGERKKRVEEIAERLGISHRLKHYP 139

Query: 134 ADLSGGQRQRVAMGRAIVRDAKVFLMDEPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVT 193
             LSGGQ+QR A+ RA+V + K+ L DEP  NLD+K    +   +  +H   GAT + VT
Sbjct: 140 QQLSGGQQQRAAVARALVNNPKIILADEPTGNLDSKNGNEVMELLTDLHAN-GATILMVT 198

Query: 194 HDQTEA 199
           H + +A
Sbjct: 199 HSEYDA 204


Lambda     K      H
   0.318    0.135    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 201
Number of extensions: 18
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 377
Length of database: 234
Length adjustment: 26
Effective length of query: 351
Effective length of database: 208
Effective search space:    73008
Effective search space used:    73008
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory