Finding step proV for L-proline catabolism in Pontibacillus litoralis JSM 072002
5 candidates for proV: proline ABC transporter, ATPase component ProV
Score | Gene | Description | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
med | N784_RS13065 | glycine betaine/L-proline ABC transporter ATP-binding protein | glycine betaine/l-proline transport atp-binding protein prov (characterized) | 50% | 99% | 373.6 | Glycine betaine/carnitine transport ATP-binding protein GbuA; EC 7.6.2.9 | 65% | 523.9 |
med | N784_RS06065 | ABC transporter ATP-binding protein | Glycine betaine/proline betaine transport system ATP-binding protein ProV (characterized) | 40% | 73% | 200.3 | BilEA aka OpuBA protein, component of A proline/glycine betaine uptake system. Also reported to be a bile exclusion system that exports oxgall and other bile compounds, BilEA/EB or OpuBA/BB (required for normal virulence) | 70% | 337.4 |
lo | N784_RS09180 | ATP-binding cassette domain-containing protein | glycine betaine/l-proline transport atp-binding protein prov (characterized) | 37% | 68% | 190.7 | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 | 43% | 250.4 |
lo | N784_RS11890 | ABC transporter ATP-binding protein | glycine betaine/l-proline transport atp-binding protein prov (characterized) | 39% | 56% | 171.8 | Uncharacterized ABC transporter ATP-binding protein YurJ, component of The arabinosaccharide transporter AraNPQMsmX. Transports α-1,5-arabinooligosaccharides, at least up to four L-arabinosyl units; the key transporter for α-1,5-arabinotriose and α-1,5-arabinotetraose, but not for α-1,5-arabinobiose which is transported by AraE. MsmX is also used by the MdxEFG-MsmX system (3.A.1.1.36) (Ferreira and Sá-Nogueira, 2010). Involved in the uptake of pectin oligosaccharides with either MsmX or YurJ as the ATPase | 51% | 357.5 |
lo | N784_RS11605 | methionine ABC transporter ATP-binding protein | Glycine betaine/proline betaine transport system ATP-binding protein ProV (characterized) | 35% | 76% | 170.6 | Methionine import ATP-binding protein MetN; EC 7.4.2.11 | 59% | 412.1 |
Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.
GapMind searches the predicted proteins for candidates by using ublast (a fast alternative to protein BLAST) to find similarities to characterized proteins or by using HMMer to find similarities to enzyme models (usually from TIGRFams). For alignments to characterized proteins (from ublast), scores of 44 bits correspond to an expectation value (E) of about 0.001.
Definition of step proV
- Curated sequence CH_001555: glycine betaine/l-proline transport atp-binding protein prov. Glycine betaine/proline betaine transport system ATP-binding protein ProV. ProV aka B2677, component of Glycine betaine/proline porter, ProU or ProVWX (also transports proline betaine, carnitine, dimethyl proline, homobetaine, γ-butyrobetaine and choline with low affinity). glycine betaine ABC transporter ATP binding subunit ProV (EC 7.6.2.9). glycine betaine ABC transporter ATP binding subunit ProV (EC 7.6.2.9)
- Curated sequence P17328: Glycine betaine/proline betaine transport system ATP-binding protein ProV
- Ignore hits to E0SCY1 when looking for 'other' hits (Glycine betaine/choline transport system ATP-binding protein OusV)
- Comment: E.coli/Salmonella have proVWX (only ProV is curated in S. typhimurium). Ignore a similar system in Dickeya dadantii (or Erwinia chrysanthemi) reported to act on glycine betaine only
Or cluster all characterized proV proteins
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory