GapMind for catabolism of small carbon sources

 

L-fucose catabolism in Halopiger salifodinae KCY07-B2

Best path

fucP, fucU, fucI, fucK, fucA, tpi, aldA

Rules

Overview: Fucose degradation in GapMind is based on the MetaCyc pathway via L-fuculose (link) or the oxidative pathway via 2,4-diketo-3-deoxy-L-fuconate (KDF) hydrolase (PMC6336799).

23 steps (13 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
fucP L-fucose:H+ symporter FucP
fucU L-fucose mutarotase FucU
fucI L-fucose isomerase FucI
fucK L-fuculose kinase FucK
fucA L-fuculose-phosphate aldolase FucA LT39_RS02315
tpi triose-phosphate isomerase LT39_RS10400 LT39_RS18880
aldA lactaldehyde dehydrogenase LT39_RS04155 LT39_RS07935
Alternative steps:
BPHYT_RS34240 ABC transporter for L-fucose, permease component
BPHYT_RS34245 ABC transporter for L-fucose, ATPase component LT39_RS09965
BPHYT_RS34250 ABC transporter for L-fucose, substrate-binding component
fdh L-fucose 1-dehydrogenase LT39_RS11815 LT39_RS00180
fucD L-fuconate dehydratase LT39_RS07820
fucDH 2-keto-3-deoxy-L-fuconate 4-dehydrogenase LT39_RS14405 LT39_RS03780
fucO L-lactaldehyde reductase LT39_RS03985 LT39_RS11060
fuconolactonase L-fucono-1,5-lactonase
HSERO_RS05250 ABC transporter for L-fucose, ATPase component LT39_RS09965
HSERO_RS05255 ABC transporter for L-fucose, permease component
HSERO_RS05260 ABC transporter for L-fucose, substrate-binding component
KDF-hydrolase 2,4-diketo-3-deoxy-L-fuconate hydrolase LT39_RS07800 LT39_RS04565
SM_b21103 ABC transporter for L-fucose, substrate-binding component
SM_b21104 ABC transporter for L-fucose, permease component 1 LT39_RS07855 LT39_RS19500
SM_b21105 ABC transporter for L-fucose, permease component 2 LT39_RS01705 LT39_RS07785
SM_b21106 ABC transporter for L-fucose, ATPase component LT39_RS16420 LT39_RS18435

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory