Align Short-chain dehydrogenase/reductase SDR (characterized, see rationale)
to candidate WP_043919846.1 jaqu_RS15175 SDR family oxidoreductase
Query= uniprot:B2T9V3 (247 letters) >NCBI__GCF_000877395.1:WP_043919846.1 Length = 246 Score = 296 bits (759), Expect = 2e-85 Identities = 153/246 (62%), Positives = 181/246 (73%), Gaps = 2/246 (0%) Query: 1 MTQRLAGKTALITAAGQGIGLATAELFAREGARVIATDIRIDGLAGKPVEARKLDVRDDA 60 M RL GK ALITAAGQGIG ATAE F EGA V ATD+ + L G E LD RDDA Sbjct: 1 MPGRLEGKRALITAAGQGIGRATAEAFVAEGAEVFATDLDLALLDGLDCETFALDARDDA 60 Query: 61 AIKALAAEIGAVDVLFNCAGFVHAGNILECSEEDWDFAFDLNVKAMYRMIRAFLPAMLDK 120 +++A + DVLFNCAGFVH G++L+ +++D+ F FDLNV +M+R IRA LP ML++ Sbjct: 61 SVRA-GVDRAQPDVLFNCAGFVHHGSVLDATDDDFAFGFDLNVHSMFRTIRAALPGMLER 119 Query: 121 GGGSIINMSSAASSVKGVPNRFAYSASKAAVIGLTKSVAADFITRGVRCNAICPGTVASP 180 GGGSI+NMSSA SSV G PNRF Y A+KAAVIGLTKSVA DFI +G+RCN ICPGTV SP Sbjct: 120 GGGSIVNMSSACSSVIGAPNRFIYGATKAAVIGLTKSVAVDFIKQGIRCNCICPGTVESP 179 Query: 181 SLEQRIVAQAQAQGATLDAVQAAFVARQPMGRIGKPEEIAALALYLGSDESSFTTGHAHV 240 S R+ A + G + D AFVARQPMGR+ EIAAL +YLGSDES+FTTGH H+ Sbjct: 180 SWHDRVKALGEEMG-SYDKAMEAFVARQPMGRVATAAEIAALVVYLGSDESAFTTGHPHI 238 Query: 241 IDGGWS 246 IDGGWS Sbjct: 239 IDGGWS 244 Lambda K H 0.320 0.133 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 200 Number of extensions: 5 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 247 Length of database: 246 Length adjustment: 24 Effective length of query: 223 Effective length of database: 222 Effective search space: 49506 Effective search space used: 49506 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory