Alignments for a candidate for dgoD in Jannaschia aquimarina GSW-M26

GapMind for catabolism of small carbon sources

Alignments for a candidate for dgoD in Jannaschia aquimarina GSW-M26

Align L-arabinonate dehydratase; ArDHT; D-fuconate dehydratase; Galactonate dehydratase; L-arabonate dehydratase; EC 4.2.1.25; EC 4.2.1.67; EC 4.2.1.6 (characterized)
to candidate WP_043918762.1 jaqu_RS09665 dihydroxy-acid dehydratase

Query= SwissProt::B5ZZ34
         (579 letters)



>NCBI__GCF_000877395.1:WP_043918762.1
          Length = 587

 Score =  282 bits (721), Expect = 3e-80
 Identities = 183/547 (33%), Positives = 287/547 (52%), Gaps = 45/547 (8%)

Query: 44  RPVIGILNTWSDMTPCNGHLRELAEKVKAGVWEAGGFPLEVPVFSASENTFRPTAMMYRN 103
           +P +G+   W++  PCN  L   A+ VK GV +A G P E    + ++        M  +
Sbjct: 41  QPFVGVATCWNEAAPCNIALNRQAQAVKLGVKQASGTPREFTTITVTDGIAMGHEGMRSS 100

Query: 104 LA-----ALAVEEAIRGQPMDGCVLLVGCDKTTPSLLMGAASCDLPSIVVTGGPMLNGYF 158
           LA     A  VE  +RG   D  V L GCDK+ P ++M     + PS+ + GG +L G  
Sbjct: 101 LASRDAIADTVELTMRGHCYDALVGLAGCDKSLPGMMMAMVRLNTPSVFIYGGSILPG-- 158

Query: 159 RGERVGSGTHL---WKFSEMV-----------KAGEMTQAEFLEAEASMSRSSGTCNTMG 204
              +  +G ++   +K  ++            +AG ++       E     S+G C    
Sbjct: 159 ---KAPAGANVPDEYKNRDLTVQDMFEAVGNHQAGNLSDEALDVLERVACPSAGACGGQF 215

Query: 205 TASTMASMAEALGMALSGNAAIPGVDSRRKVMAQLTGRRIVQMVKDDLKPSEIMTKQAFE 264
           TA+TMA ++EA+G+AL  ++  P     R   +  +G  ++++++ +++  +I+T+++ E
Sbjct: 216 TANTMACVSEAIGLALPNSSGAPAPYESRDQYSIASGEAVMRLLEKNIRARDIVTRKSLE 275

Query: 265 NAIRTNAAIGGSTNAVIHLLAIAGRVGIDLSLDDWDRCGRDVPTIVNLMPSGKYLMEEFF 324
           NA R  A  GGSTNA +HL AIA   GID  L D     RD P  V+L P G+Y+ ++ +
Sbjct: 276 NAARVVACTGGSTNAGLHLPAIAHEAGIDFDLFDVCEIFRDTPYFVDLKPGGQYVAKDLY 335

Query: 325 YAGGLPVVLKRLGEAGLLHKDALTVSGETVWDEVKDVVNWNED--VILPAEKALTSSGGI 382
            AGG+PVV+K L +AGL+H+D +T +G+T+  EV D V    D  VI P +  +T +GG+
Sbjct: 336 EAGGVPVVMKELRKAGLIHEDCITATGKTI-GEVLDEVKGEADGKVIYPVDAPITPTGGV 394

Query: 383 VVLRGNLAPKGAVLKPSAASPHLLVHKGRAVVFEDIDDYKAKINDDNLDIDENCIMVMKN 442
           V L+GNLAP GA++K +          G A VFE  +D  A +       +E  ++V++N
Sbjct: 395 VGLKGNLAPDGAIVKVAGIPSQHQRFTGPARVFECEEDAFAAVK--ARAYEEGEVLVIRN 452

Query: 443 CGPKGYPGMAE-------VGNMGLPPKVLKKGILDMVRISDARMSGTAYGTVVLHTSPEA 495
            GP G PGM E       +   G+  KV          I+D R SG   G  V H  PEA
Sbjct: 453 EGPAGGPGMREMLATTAALSGQGMGKKV--------ALITDGRFSGATRGFCVGHVGPEA 504

Query: 496 AVGGPLAVVKNGDMIELDVPNRRLHLDISDEELARRLAEWQ-PNHDLPTSGYAFLHQQHV 554
           A GGP+ ++++GD+I +D     L +D+++EEL  R A+W+ P   +  SG  + + + V
Sbjct: 505 AHGGPIGMLRDGDVITIDAIKGELSVDLTEEELVARKADWKGPRETIYASGALWKYARLV 564

Query: 555 EGADTGA 561
             A TGA
Sbjct: 565 GPARTGA 571


Lambda     K      H
   0.318    0.135    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 931
Number of extensions: 54
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 579
Length of database: 587
Length adjustment: 36
Effective length of query: 543
Effective length of database: 551
Effective search space:   299193
Effective search space used:   299193
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources