GapMind for catabolism of small carbon sources

 

myo-inositol catabolism in Jannaschia aquimarina GSW-M26

Best path

iolT, iolG, iolM, iolN, iolO, uxaE, uxuB, uxuA, kdgK, eda

Rules

Overview: Myo-inositol degradation in GapMind is based on MetaCyc pathways myo-inositol degradation I via inosose dehydratase (link) and pathway II inosose dehydrogenase (link).

29 steps (13 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
iolT myo-inositol:H+ symporter
iolG myo-inositol 2-dehydrogenase jaqu_RS20480 jaqu_RS07950
iolM 2-inosose 4-dehydrogenase
iolN 2,4-diketo-inositol hydratase
iolO 5-dehydro-L-gluconate epimerase
uxaE D-tagaturonate epimerase
uxuB D-mannonate dehydrogenase jaqu_RS16445 jaqu_RS12405
uxuA D-mannonate dehydratase jaqu_RS16440
kdgK 2-keto-3-deoxygluconate kinase jaqu_RS16435
eda 2-keto-3-deoxygluconate 6-phosphate aldolase jaqu_RS06440 jaqu_RS18695
Alternative steps:
HMIT myo-inositol:H+ symporter
iatA myo-inositol ABC transporter, ATPase component IatA jaqu_RS16085 jaqu_RS12955
iatP myo-inositol ABC transporter, permease component IatP jaqu_RS12915 jaqu_RS12950
ibpA myo-inositol ABC transporter, substrate-binding component IbpA
iolB 5-deoxy-D-glucuronate isomerase
iolC 5-dehydro-2-deoxy-D-gluconate kinase
iolD 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase
iolE scyllo-inosose 2-dehydratase
iolF myo-inositol:H+ symporter
iolJ 5-dehydro-2-deoxyphosphogluconate aldolase
mmsA malonate-semialdehyde dehydrogenase jaqu_RS11085 jaqu_RS03630
PGA1_c07300 myo-inositol ABC transport, substrate-binding component
PGA1_c07310 myo-inositol ABC transporter, permease component
PGA1_c07320 myo-inositol ABC transporter, ATPase component jaqu_RS18970 jaqu_RS07625
PS417_11885 myo-inositol ABC transporter, substrate-binding component jaqu_RS07615
PS417_11890 myo-inositol ABC transporter, ATPase component jaqu_RS16085 jaqu_RS12955
PS417_11895 myo-inositol ABC transporter, permease component jaqu_RS12915 jaqu_RS12950
SMIT1 myo-inositol:Na+ symporter
tpi triose-phosphate isomerase jaqu_RS04910 jaqu_RS05175

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory