Alignments for a candidate for sdlC in Photobacterium gaetbulicola Gung47

GapMind for catabolism of small carbon sources

Alignments for a candidate for sdlC in Photobacterium gaetbulicola Gung47

Align Dicarboxylate (succinate, fumarate, malate) transporter, vcINDY (characterized)
to candidate WP_044623788.1 H744_RS21915 SLC13/DASS family transporter

Query= TCDB::Q9KNE0
         (462 letters)



>NCBI__GCF_000940995.1:WP_044623788.1
          Length = 471

 Score =  173 bits (439), Expect = 1e-47
 Identities = 136/444 (30%), Positives = 227/444 (51%), Gaps = 46/444 (10%)

Query: 46  VLGISMLAFIAVLWLTEALHVTVTAILVPVMAVFF----GIFETQAA------------- 88
           V+ I +LA  A+ W+ E + +  T++++ V+ +      G++  + A             
Sbjct: 37  VIAIFLLA--ALCWVLEPIPIYATSVVIIVLELMLLSDKGLYLFRGAEGQPHFGELLNYS 94

Query: 89  --LNNFANSIIFLFLGGFALAAAMHHQGLDKVIADKVLAMAQGKMSVAVFMLFGVTALLS 146
             +  FA+ II LFLGGF LA A     LD  +A  +L     +    +F L  +TA+ S
Sbjct: 95  DIMATFASPIIMLFLGGFFLAMAATKYRLDVNLARVLLRPFGTQPKYVMFGLMLITAIFS 154

Query: 147 MWISNTATAAMMLPLVLGVLSKVDADKQRSTYVFVLLGVAYSASIGGIATLVGSPPNAIA 206
           M++SNTAT AMML ++  V++   A K      F L  +  +A+IGGI T +G+PPNAIA
Sbjct: 155 MFMSNTATTAMMLSILAPVIALFGA-KDPGKIAFALC-IPVAANIGGIGTPIGTPPNAIA 212

Query: 207 ----AAEVGLSFTDWMKFGLPTAMMMLPMAIAILYFLLKPTLNGMFELDRAPVNWDKGKV 262
                 +  ++F +WM FG+P   +ML  A  +L   L P      EL          K 
Sbjct: 213 LKYLTGDNMITFGEWMFFGVPFVAVMLVFA-WVLINALYPAKQEKIELTIKGKFLMTPKA 271

Query: 263 VTLGI-FGLTVFLWIFSSPINAALGGFKSFDTLVALGAILMLSFARVVHWKEIQKTADWG 321
           +T+ + F LT+ LW+  S       G  S+   VAL  + + S   +++ KE  K   W 
Sbjct: 272 ITVYVTFALTIILWLMGS-----AHGMNSY--TVALIPVAVFSLTGIIN-KEDLKKISWD 323

Query: 322 VLLLFGGGLCLSNVLKQTGTSVFLANALSDMVSHMGIFVVILVVATFVVFLTEFASNTAS 381
           VL L  GG+ L   L QTG +  + +++         ++V+   A   + +  F S+TA+
Sbjct: 324 VLWLVSGGIALGLALDQTGLARLMVHSIP--FDSFSPYIVLGGSALLCLVMANFMSHTAT 381

Query: 382 AALLIPVFATVAEAF-GMSP----VLLSVLIAVAASCAFMLPVATPPNAIVFASGHIKQS 436
           A LL+P+ A +  +   + P    + L +++  AAS    LP++TPPNA+  A+GH++ +
Sbjct: 382 ANLLMPIMAALGTSMVSLVPLGGEITLILVVTFAASLGMSLPISTPPNALAHATGHVQSN 441

Query: 437 EMMRVGLYLNIACIGLLTAIAMLF 460
           +M RVG+ + +  +G++ + AM++
Sbjct: 442 QMARVGVIIGV--VGVVLSFAMIW 463



 Score = 55.5 bits (132), Expect = 4e-12
 Identities = 43/193 (22%), Positives = 97/193 (50%), Gaps = 12/193 (6%)

Query: 53  AFIAVLWLTEALHV--TVTAILVPVMAVFFGIFETQAALNNFANSIIFLFLGGFALAAAM 110
           A   +LWL  + H   + T  L+PV          +  L   +  +++L  GG AL  A+
Sbjct: 279 ALTIILWLMGSAHGMNSYTVALIPVAVFSLTGIINKEDLKKISWDVLWLVSGGIALGLAL 338

Query: 111 HHQGLDKVIADKVLAMAQGKMSVAVFMLFGVTALLSM----WISNTATAAMMLPLVLGVL 166
              GL +++   +        S + +++ G +ALL +    ++S+TATA +++P++  + 
Sbjct: 339 DQTGLARLMVHSIPFD-----SFSPYIVLGGSALLCLVMANFMSHTATANLLMPIMAALG 393

Query: 167 SKVDADKQRSTYVFVLLGVAYSASIGGIATLVGSPPNAIAAAEVGLSFTDWMKFGLPTAM 226
           + + +       + ++L V ++AS+G ++  + +PPNA+A A   +      + G+   +
Sbjct: 394 TSMVSLVPLGGEITLILVVTFAASLG-MSLPISTPPNALAHATGHVQSNQMARVGVIIGV 452

Query: 227 MMLPMAIAILYFL 239
           + + ++ A+++ L
Sbjct: 453 VGVVLSFAMIWLL 465


Lambda     K      H
   0.329    0.139    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 616
Number of extensions: 44
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 3
Number of HSP's successfully gapped: 2
Length of query: 462
Length of database: 471
Length adjustment: 33
Effective length of query: 429
Effective length of database: 438
Effective search space:   187902
Effective search space used:   187902
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources