Alignments for a candidate for nagF in Chromobacterium vaccinii MWU205

GapMind for catabolism of small carbon sources

Alignments for a candidate for nagF in Chromobacterium vaccinii MWU205

Align N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) (characterized)
to candidate WP_046156459.1 VL52_RS07480 phosphoenolpyruvate--protein phosphotransferase

Query= reanno::BFirm:BPHYT_RS02740
         (854 letters)



>NCBI__GCF_000971335.1:WP_046156459.1
          Length = 830

 Score =  343 bits (879), Expect = 3e-98
 Identities = 233/667 (34%), Positives = 337/667 (50%), Gaps = 22/667 (3%)

Query: 189 GLHARPAARAREAARGFDARVEVRYE--GRKAAIESVVGLLG--LGAGEGATVELLGMGP 244
           G+HARPA+R  E   GF A +  R +  G+ A  +SV+ L+   +   +   +E+ G G 
Sbjct: 14  GIHARPASRIAEFCAGFGAAIRWRNQRNGQWADAKSVLSLIAGDILFNDVVDIEIDGEG- 72

Query: 245 QAAAAVAAIANELTREAHGEVE--EKPARQSSPAPQAVARPAGETLAPNTLAGVCAAPGV 302
                 A +A+EL R     +   ++P   +  A +A+ R   E L+P+   G  A  GV
Sbjct: 73  -----AARVADELGRFLRQTLPCCDEPVAAAPAASRALPRSLAE-LSPSPCFGQPAGQGV 126

Query: 303 AVGKLVRWDDADIDPPEKANGTSAAESRLLDKAIATVDADLDTTVRDASQRGAVGEAGIF 362
           A+G+L       +  P +   ++  ++ L   A+A     L   +R  ++      AG+ 
Sbjct: 127 AIGELCVHRGFRMPEPAELPLSTDRDADLA--ALAVARRTLAEQLRQQAEHQGGEAAGVS 184

Query: 363 SVHRVLLEDPTL---LDAARDLISLGKSAGFAWREAIRAQIAILTNIEDALLAERAADLR 419
             H  +L DP     LDAA   ++ G     A    +    A L+      L ER  D+R
Sbjct: 185 RAHLAILSDPDFRARLDAA---VADGAGPARAVAVVVAHYRAQLSKSASEYLREREMDIR 241

Query: 420 DIEKRVLRALGYTSATA-RTLPEEAVLAAEEFTPSDLSTLDRSRVTALVMARGGATSHAA 478
           D+ +R+L AL      A   LPE A+L AE+  PS+L  LDR +V  L++ARGG TSH  
Sbjct: 242 DVGRRLLEALAPEIQRAGEALPEAALLWAEDLAPSELLALDRDKVKGLLLARGGLTSHTI 301

Query: 479 ILARQAGIPALVAVGDALHAIPEGTQVVVNATTGRLEFAPTELDVERARLERTRLADVRE 538
           ILAR   +P L  V  A  A   G   V++A  G L  +P  + ++  R E   LA  R+
Sbjct: 302 ILARAFSLPVLTGVQRAAIADGIGRTAVLDAGLGALFQSPDTVVLDYYREEEALLAAQRQ 361

Query: 539 ANRRTSQQAAVTSDGRAIEVAANIATLDDAKTAVENGADSVGLLRTELLFIHRAAAPTTD 598
              + S   A ++DG  +E+A NIA+  +A+     GAD VGL RTE+LF+     P  D
Sbjct: 362 RLAKASGGIAASADGHRLELAVNIASAAEAQQGFAQGADGVGLFRTEMLFMDADEPPGED 421

Query: 599 EHRQSYQAIVDALSGRTAIIRTLDVGADKEVDYLTLPPEPNPALGLRGIRLAQVRPDLLD 658
           E  Q Y  +V   +GR  IIRT D+G DK    L  P E NP LG R IR+   +     
Sbjct: 422 EQFQCYAEVVRLAAGRPVIIRTFDIGGDKPARCLPAPQESNPFLGYRAIRMYPDQEAAFR 481

Query: 659 DQLRGLLAVQPLGAVRILLPMVTDVGELIRIRKRIDEFARELGRTEPIEVGVMIEVPSAA 718
            QLR +L    LG V++++PMV  +GE    R+ ++E    LGR EP+ +G+M+EVPS  
Sbjct: 482 AQLRAILRASALGPVKVMIPMVATLGEARWARRLLEEERAALGRAEPVPLGIMLEVPSVL 541

Query: 719 LLADQLAQHADFLSIGTNDLTQYTLAMDRCQADLAAQADGLHPAVLRLIAATVQGADKHG 778
              D   +  DF S+G+NDLTQY  A DR    +    D LHPA L  +        +HG
Sbjct: 542 FQLDAFCREVDFFSVGSNDLTQYLFAADRDNERVGNLYDSLHPAFLAALNQAAATIHRHG 601

Query: 779 KWVGVCGALAGDPLAMPLLVGLGVTELSVDPVSVPGIKARVRNLDYQLCRQRAQDALALE 838
           +W+G+CG  A  P A+PL +G+G+ ELS+   S+   + R+R LD   CR+    ALA  
Sbjct: 602 RWIGLCGEAAAAPHALPLFLGMGLDELSMSAPSLQECRRRLRELDADRCRELLGRALACA 661

Query: 839 SAQAVRA 845
               VRA
Sbjct: 662 DGAEVRA 668


Lambda     K      H
   0.317    0.133    0.371 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1563
Number of extensions: 75
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 854
Length of database: 830
Length adjustment: 42
Effective length of query: 812
Effective length of database: 788
Effective search space:   639856
Effective search space used:   639856
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources