Alignments for a candidate for fadB in Chromobacterium vaccinii MWU205

GapMind for catabolism of small carbon sources

Alignments for a candidate for fadB in Chromobacterium vaccinii MWU205

Align 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); enoyl-CoA hydratase (EC 4.2.1.17) (characterized)
to candidate WP_046156967.1 VL52_RS10360 3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase family protein

Query= BRENDA::Q0KEG0
         (807 letters)



>NCBI__GCF_000971335.1:WP_046156967.1
          Length = 791

 Score =  924 bits (2387), Expect = 0.0
 Identities = 475/808 (58%), Positives = 590/808 (73%), Gaps = 22/808 (2%)

Query: 2   SNFIVKKVAVLGAGVMGAQIAAHLINARVPVVLFDLPAKEGPKNGIALRAIENLKKLSPA 61
           + F V+KVAVLGAGVMGAQIAAHL+NA+VP +LFDLPAKEG KNGIAL+AI+ LKKL P+
Sbjct: 4   TKFNVRKVAVLGAGVMGAQIAAHLVNAKVPTILFDLPAKEGDKNGIALKAIDGLKKLKPS 63

Query: 62  PLGIKEEAGLIQAANYEDDIALLKECDLVIEAIAERMDWKHDLYKKVAPHLASHAIFATN 121
           PL   +    IQ ANY+D + LLK+CDLVIEAIAERMDWK DLY KVAPHL  H IFATN
Sbjct: 64  PLAGSDVVAHIQPANYDDHLHLLKDCDLVIEAIAERMDWKVDLYHKVAPHLGEHTIFATN 123

Query: 122 TSGLSITALSDGFDADLKSRFCGVHFFNPPRYMHLVELIPTATTQPQILDQLEAFLTTTL 181
           TSGLSI  L++G    ++ RFCGVHFFNPPRYMHLVE+IP  T+   ILD LE FL ++L
Sbjct: 124 TSGLSINKLAEGCPDSVRPRFCGVHFFNPPRYMHLVEIIPCVTSDAHILDNLERFLVSSL 183

Query: 182 GKGVVRAKDTPNFIANRVGIFSILAVFAEAEKFGIPFDVVDDLTGSKLGRAKSATFRTAD 241
           GKGVVRAKDTPNF+ANR+G+FS+LA  A A K+GI FDVVDDLTG +LGR KSATFRTAD
Sbjct: 184 GKGVVRAKDTPNFVANRIGVFSMLATIANAAKYGIRFDVVDDLTGPRLGRPKSATFRTAD 243

Query: 242 VVGLDTMAHVIKTMQDTLHDDPFAPVYKTPAVLKGLVDAGALGQKTGAGFYKKEGKAIKV 301
           VVGLDT AHV+KTMQDTL  DP+  ++ TP  ++ L+  GALG KT AG YKK+GK + V
Sbjct: 244 VVGLDTFAHVVKTMQDTLPQDPWHSLFATPDWMQQLIANGALGAKTRAGIYKKDGKRMLV 303

Query: 302 LDAKTGQYVDAGKKADEIVVRMLK-KDAAERIKLLRESTNPQAQFLWAVFRDVFHYIAVY 360
           LD   G+YV +G+K D+ V  +LK  D AE+ + LR+S +PQAQFLWA FRDVFHYI+ +
Sbjct: 304 LDPAKGEYVGSGEKGDDAVKDILKISDPAEKFQKLRDSEHPQAQFLWACFRDVFHYISFH 363

Query: 361 LEQIAGSAADIDLAIRWGFGWNSGPFEDWQSAGWKQVAEWVKEDVEAGKALSAAPLPAWV 420
           L  IA  A D+D AIRWGFGW++GPFE WQSAGW+QVA+W+KED+ AGK L+ A LPAWV
Sbjct: 364 LGDIANCARDVDFAIRWGFGWSAGPFETWQSAGWQQVAKWLKEDIAAGKTLAKAELPAWV 423

Query: 421 FEGPVAENQGVHAAAGSWSPATQSFVARSKLPVYQRQAFRAAIKGTAAADPRKAGRTVEE 480
            EG   +  GVH A GS++ A +  V RS L VYQRQ   A + G  +      G TV E
Sbjct: 424 LEG---DRAGVHFADGSYNAADKKLVGRSTLDVYQRQLAPAKVLGEKS---ELLGETVFE 477

Query: 481 NDAVRIWVSEGQDDVLVVSFKSKMNTIGPDVIDGLTRAIDLAEAGYKGLVVWQPTSLQLG 540
           N+ VR + +   D+VLVVSFKSK + IGP V+DGL  A+D+AE  +KGLV+WQ       
Sbjct: 478 NEGVRAFTT--GDEVLVVSFKSKAHAIGPAVLDGLNTALDIAEDRFKGLVIWQTEE---- 531

Query: 541 APGGPFSAGANLEAAMPAFMMGGAKGIEPFVKRFQDGMMRVKYASVPVVSAASGIALGGG 600
               PFS GA+L++ MPAFMMG    I+  + RFQ   +R++Y+ VP V+A  G   GGG
Sbjct: 532 ----PFSVGADLQSMMPAFMMGDWDAIDAMIGRFQSTALRLRYSQVPTVAATQGYVFGGG 587

Query: 601 CELMLHSASRVAALETYIGLVEVGVGLVPAGGGLKEAALAAARAAQAAGSTNILQFLTSR 660
           CE  +H    VAALE+Y+GLVEVGVGL+P GGG KE AL A++ ++     ++L  L   
Sbjct: 588 CEFAMHCDKTVAALESYVGLVEVGVGLLPGGGGCKEFALRASQESKG----DLLAALKDY 643

Query: 661 FQSAAMAKVSASALEARQMGYLQPSDKIVFNVHELLYVAQNEVRALASAGYRAPLPTL-V 719
           F + A AKV+ S +EA+++G+ +  D +VFN +ELLYVA+ +  ALA +GYR PL     
Sbjct: 644 FMNIATAKVATSGVEAQELGFFRKGDPVVFNAYELLYVAKKQALALAESGYRPPLKVKGF 703

Query: 720 PVAGRSGIATIKASLVNMRDGGFISTHDFLIASRIAEAVCGGDVEAGSLVSEDWLLALER 779
           PVAGRSG A+IK  LVNM +G FIS HDF IAS+IA+ + GGD+EAG+LV+E W+L LER
Sbjct: 704 PVAGRSGAASIKGQLVNMLEGNFISQHDFFIASQIADVMTGGDLEAGTLVNEQWILELER 763

Query: 780 KAFVDLLGTGKTQERIMGMLQTGKPVRN 807
           KAF+ LL   KTQ+RI  ML TGKP+RN
Sbjct: 764 KAFMTLLKNSKTQDRIANMLTTGKPLRN 791


Lambda     K      H
   0.319    0.134    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1551
Number of extensions: 55
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 807
Length of database: 791
Length adjustment: 41
Effective length of query: 766
Effective length of database: 750
Effective search space:   574500
Effective search space used:   574500
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources