GapMind for catabolism of small carbon sources

 

Protein WP_047006204.1 in Erythrobacter gangjinensis K7-2

Annotation: NCBI__GCF_001010925.1:WP_047006204.1

Length: 426 amino acids

Source: GCF_001010925.1 in NCBI

Candidate for 8 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
D-glucuronate catabolism dctM hi Putative TRAP dicarboxylate transporter, DctM subunit (characterized, see rationale) 45% 99% 367.5 Sialic acid TRAP transporter large permease protein SiaM 40% 300.4
D-gluconate catabolism gntB lo D-gluconate TRAP transporter, large permease component (characterized) 37% 98% 290 Sialic acid TRAP transporter large permease protein SiaM 40% 300.4
L-malate catabolism dctM lo C4-dicarboxylate TRAP transporter large permease protein DctM (characterized) 38% 99% 287.7 Sialic acid TRAP transporter large permease protein SiaM 40% 300.4
fumarate catabolism dctM lo C4-dicarboxylate TRAP transporter large permease protein DctM (characterized) 38% 99% 287.7 Sialic acid TRAP transporter large permease protein SiaM 40% 300.4
succinate catabolism dctM lo C4-dicarboxylate TRAP transporter large permease protein DctM (characterized) 38% 99% 287.7 Sialic acid TRAP transporter large permease protein SiaM 40% 300.4
2-oxoglutarate (alpha-ketoglutarate) catabolism dctM lo dicarboxylate TRAP transporter (succinate, fumarate, L-malate, and alpha-ketoglutarate), large permease component (characterized) 33% 99% 255.4 Sialic acid TRAP transporter large permease protein SiaM 40% 300.4
L-glutamate catabolism gtrB lo GtrB aka SLL1103, component of Tripartite glutamate:Na+ symporter (characterized) 32% 96% 201.8 Sialic acid TRAP transporter large permease protein SiaM 40% 300.4
pyruvate catabolism dctM lo Alr3027 protein, component of The 2-oxo monocarboxylate transporter (Pernil et al., 2010). Transports pyruvate which is inhibited by various 2-ketoacids (characterized) 31% 96% 201.1 Sialic acid TRAP transporter large permease protein SiaM 40% 300.4

Sequence Analysis Tools

View WP_047006204.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MELTVLLLLLAVLLAIGVPVAFALGAASVATFLLLDIPVVVAFQRMAASMNVFTLMAIPF
FVFAGDLMARTGIAERLVRVAEGAFGRARGGLGQVDVGASMMFGAVSGSAVASVSAMGST
LMPMMKEKGYDADYAVNVTITAAILGILIPPSHNMIIYAAASPVSVSVGDLFLAGIVPGL
LAGAALMFVAWRVAIRRGYPGGTFPGWRSFTKSLIAAFPGLMTALIIVFGILLGVFTPTE
SSAVAVIYTIVIGVLVYRSLGYIAFVEAASSAVRTTAMVMLIIGTAGMFGWLFALLHGPE
MLSGGLTAVSQDPAVIMLMILLVLLVLGAFMDMAPLIIITTPIFLPVAVATGVDPVHFGI
IMMLSLGIGLVTPPVGSVLFVGCAVGQAKPEQVVRTIWPFYLALMIVLLLVAYLPSLSLW
LPEQFN

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory