GapMind for catabolism of small carbon sources

 

Alignments for a candidate for glcV in Erythrobacter gangjinensis K7-2

Align monosaccharide-transporting ATPase (EC 3.6.3.17) (characterized)
to candidate WP_047007399.1 AAW01_RS11525 ABC transporter ATP-binding protein

Query= BRENDA::Q97UY8
         (353 letters)



>NCBI__GCF_001010925.1:WP_047007399.1
          Length = 255

 Score =  124 bits (312), Expect = 2e-33
 Identities = 77/203 (37%), Positives = 114/203 (56%), Gaps = 10/203 (4%)

Query: 4   IIVKNVSKVFKKGKVV--ALDNVNINIENGERFGILGPSGAGKTTFMRIIAGLDVPSTGE 61
           I +++++K F  G     AL  V++ IE G+   ++GPSG+GK+T M I+  LDVP++G 
Sbjct: 22  IELRDITKTFGTGAAAFQALKGVDMTIERGDFVAVMGPSGSGKSTTMNILGCLDVPTSGT 81

Query: 62  LYFD----DRLVASNGKLIVPPEDRKIGMVFQTWALYPNLTAFENIAFPLTNMKMSKEEI 117
             F       L      L+     R +G VFQ + L    +A EN+  PL     +K++ 
Sbjct: 82  FRFRGVEVQDLSRDQRSLL---RRRYLGFVFQGFNLLARTSALENVELPLLYRGETKKQR 138

Query: 118 RKRVEEVAKILDIHHVLNHFPRELSGGQQQRVALARALVKDPSLLLLDEPFSNLDARMRD 177
           R+  E    ++ +    +H P ELSGGQQQRVA+ARALV DP +LL DEP  NLD     
Sbjct: 139 REAAERSLDLVGLLPWADHTPAELSGGQQQRVAIARALVTDPDVLLADEPTGNLDTERSV 198

Query: 178 SARALVKEVQSRLGVTLLVVSHD 200
               L+ E+  R G+T+L+V+H+
Sbjct: 199 EIMELLTELNQR-GITVLMVTHE 220


Lambda     K      H
   0.319    0.139    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 171
Number of extensions: 8
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 353
Length of database: 255
Length adjustment: 27
Effective length of query: 326
Effective length of database: 228
Effective search space:    74328
Effective search space used:    74328
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory