Align GluA aka CGL1950, component of Glutamate porter (characterized)
to candidate WP_047006469.1 AAW01_RS01810 phosphate ABC transporter ATP-binding protein
Query= TCDB::P48243 (242 letters) >NCBI__GCF_001010925.1:WP_047006469.1 Length = 280 Score = 141 bits (355), Expect = 2e-38 Identities = 88/245 (35%), Positives = 136/245 (55%), Gaps = 12/245 (4%) Query: 2 IKMTGVQKYFGDFHALTDIDLEIPRGQVVVVLGPSGSGKSTLCRTINRL-ETIE----EG 56 ++ GV Y+GD A+ D+ ++I V +GPSG GKST R +NR+ +TI +G Sbjct: 33 MRAQGVDVYYGDKRAIDDVSIDIYTEYVTAFIGPSGCGKSTFLRALNRMNDTIPSASVKG 92 Query: 57 TIEIDGKVLPEEGKGLANLRADVGMVFQSFNLFPHLTIKDNVTLAP-IKVRKMKKSEAEK 115 IE+DG + G + LRA VGMVFQ N FP +I +N+ P I +++ ++ Sbjct: 93 RIELDGDDIYASGMDVVQLRARVGMVFQKPNPFPK-SIYENIAYGPRIHGLAENRNDVDE 151 Query: 116 LAMSLLERVG----IANQADKYPAQLSGGQQQRVAIARALAMNPKIMLFDEPTSALDPEM 171 + L R G + ++ LSGGQQQR+ IARA+A++P+++L DEP SALDP Sbjct: 152 IVEKSLRRAGLWEEVKDRLQDSGTALSGGQQQRLCIARAIAVDPEVILMDEPCSALDPIA 211 Query: 172 VNEVLDVMASLAKEGMTMVCVTHEMGFARKAADRVLFMADGLIVEDTEPDSFFTNPKSDR 231 ++ +++ L + +V VTH M A + + R F G +VE FTNP+ R Sbjct: 212 TAKIEELIDEL-RGRYGIVIVTHSMQQAARVSQRTAFFHLGKMVEYGHTSDIFTNPREKR 270 Query: 232 AKDFL 236 +D++ Sbjct: 271 TQDYI 275 Lambda K H 0.319 0.135 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 155 Number of extensions: 11 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 242 Length of database: 280 Length adjustment: 24 Effective length of query: 218 Effective length of database: 256 Effective search space: 55808 Effective search space used: 55808 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory