Protein WP_047092042.1 in Erythrobacter marinus HWDM-33
Annotation: NCBI__GCF_001013305.1:WP_047092042.1
Length: 233 amino acids
Source: GCF_001013305.1 in NCBI
Candidate for 43 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| L-lysine catabolism | hisP | med | Amino-acid ABC transporter, ATP-binding protein (characterized, see rationale) | 41% | 82% | 139.4 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| L-arginine catabolism | artP | med | Arginine transport ATP-binding protein ArtP; EC 7.4.2.- (characterized) | 41% | 86% | 135.6 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| L-histidine catabolism | PA5503 | lo | Methionine import ATP-binding protein MetN 2, component of L-Histidine uptake porter, MetIQN (characterized) | 37% | 68% | 143.7 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| L-asparagine catabolism | glnQ | lo | Glutamine ABC transporter ATP-binding protein, component of Glutamine transporter, GlnQP. Takes up glutamine, asparagine and glutamate which compete for each other for binding both substrate and the transmembrane protein constituent of the system (Fulyani et al. 2015). Tandem substrate binding domains (SBDs) differ in substrate specificity and affinity, allowing cells to efficiently accumulate different amino acids via a single ABC transporter. Analysis revealed the roles of individual residues in determining the substrate affinity (characterized) | 38% | 87% | 140.6 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| L-glutamate catabolism | gltL | lo | Glutamine ABC transporter ATP-binding protein, component of Glutamine transporter, GlnQP. Takes up glutamine, asparagine and glutamate which compete for each other for binding both substrate and the transmembrane protein constituent of the system (Fulyani et al. 2015). Tandem substrate binding domains (SBDs) differ in substrate specificity and affinity, allowing cells to efficiently accumulate different amino acids via a single ABC transporter. Analysis revealed the roles of individual residues in determining the substrate affinity (characterized) | 38% | 87% | 140.6 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| D-glucosamine (chitosamine) catabolism | AO353_21725 | lo | ABC transporter for D-Glucosamine, putative ATPase component (characterized) | 36% | 89% | 138.3 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| L-histidine catabolism | bgtA | lo | BgtA aka SLR1735, component of Arginine/lysine/histidine/glutamine porter (characterized) | 36% | 90% | 137.5 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| L-asparagine catabolism | aatP | lo | Glutamate/aspartate transport ATP-binding protein GltL aka B0652, component of Glutamate/aspartate porter (characterized) | 36% | 90% | 132.1 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| L-aspartate catabolism | aatP | lo | Glutamate/aspartate transport ATP-binding protein GltL aka B0652, component of Glutamate/aspartate porter (characterized) | 36% | 90% | 132.1 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| D-maltose catabolism | thuK | lo | ThuK aka RB0314 aka SMB20328, component of Trehalose/maltose/sucrose porter (trehalose inducible) (characterized) | 37% | 61% | 130.2 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| sucrose catabolism | thuK | lo | ThuK aka RB0314 aka SMB20328, component of Trehalose/maltose/sucrose porter (trehalose inducible) (characterized) | 37% | 61% | 130.2 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| trehalose catabolism | thuK | lo | ThuK aka RB0314 aka SMB20328, component of Trehalose/maltose/sucrose porter (trehalose inducible) (characterized) | 37% | 61% | 130.2 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| L-fucose catabolism | SM_b21106 | lo | ABC transporter for L-Fucose, ATPase component (characterized) | 38% | 55% | 128.3 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| D-maltose catabolism | malK_Bb | lo | ABC-type maltose transport, ATP binding protein (characterized, see rationale) | 36% | 60% | 127.5 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| lactose catabolism | lacK | lo | LacK, component of Lactose porter (characterized) | 34% | 58% | 127.1 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| D-sorbitol (glucitol) catabolism | mtlK | lo | ABC transporter for D-Sorbitol, ATPase component (characterized) | 37% | 56% | 127.1 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| putrescine catabolism | potA | lo | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 (characterized) | 35% | 58% | 124.4 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| L-histidine catabolism | hutV | lo | ABC transporter for L-Histidine, ATPase component (characterized) | 39% | 78% | 123.6 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| N-acetyl-D-glucosamine catabolism | SMc02869 | lo | N-Acetyl-D-glucosamine ABC transport system, ATPase component (characterized) | 38% | 60% | 123.2 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| D-glucosamine (chitosamine) catabolism | SMc02869 | lo | N-Acetyl-D-glucosamine ABC transport system, ATPase component (characterized) | 38% | 60% | 123.2 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| D-mannitol catabolism | mtlK | lo | SmoK aka POLK, component of Hexitol (glucitol; mannitol) porter (characterized) | 36% | 63% | 122.5 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| D-cellobiose catabolism | aglK' | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 37% | 57% | 122.1 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| D-glucose catabolism | aglK' | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 37% | 57% | 122.1 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| lactose catabolism | aglK' | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 37% | 57% | 122.1 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| D-maltose catabolism | aglK | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 37% | 57% | 122.1 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| D-maltose catabolism | aglK' | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 37% | 57% | 122.1 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| sucrose catabolism | aglK | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 37% | 57% | 122.1 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| sucrose catabolism | aglK' | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 37% | 57% | 122.1 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| trehalose catabolism | aglK | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 37% | 57% | 122.1 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| trehalose catabolism | aglK' | lo | Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) | 37% | 57% | 122.1 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| D-cellobiose catabolism | gtsD | lo | GtsD (GLcK), component of Glucose porter, GtsABCD (characterized) | 35% | 55% | 118.6 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| D-glucose catabolism | gtsD | lo | GtsD (GLcK), component of Glucose porter, GtsABCD (characterized) | 35% | 55% | 118.6 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| lactose catabolism | gtsD | lo | GtsD (GLcK), component of Glucose porter, GtsABCD (characterized) | 35% | 55% | 118.6 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| D-maltose catabolism | gtsD | lo | GtsD (GLcK), component of Glucose porter, GtsABCD (characterized) | 35% | 55% | 118.6 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| sucrose catabolism | gtsD | lo | GtsD (GLcK), component of Glucose porter, GtsABCD (characterized) | 35% | 55% | 118.6 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| trehalose catabolism | gtsD | lo | GtsD (GLcK), component of Glucose porter, GtsABCD (characterized) | 35% | 55% | 118.6 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| trehalose catabolism | treV | lo | TreV, component of Trehalose porter (characterized) | 34% | 60% | 118.2 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| L-proline catabolism | proV | lo | glycine betaine/l-proline transport atp-binding protein prov (characterized) | 34% | 51% | 117.1 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| D-galactose catabolism | PfGW456L13_1897 | lo | ABC transporter for D-Galactose and D-Glucose, ATPase component (characterized) | 34% | 55% | 115.9 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| L-arabinose catabolism | xacK | lo | Xylose/arabinose import ATP-binding protein XacK; EC 7.5.2.13 (characterized, see rationale) | 36% | 56% | 114 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| D-maltose catabolism | malK | lo | Maltose-transporting ATPase (EC 3.6.3.19) (characterized) | 38% | 53% | 113.6 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| D-cellobiose catabolism | TM0027 | lo | TM0027, component of β-glucoside porter (Conners et al., 2005). Binds cellobiose, laminaribiose (Nanavati et al. 2006). Regulated by cellobiose-responsive repressor BglR (characterized) | 32% | 73% | 88.6 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
| D-cellobiose catabolism | cbtF | lo | CbtF, component of Cellobiose and cellooligosaccharide porter (characterized) | 30% | 63% | 88.6 | Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- | 44% | 188.3 |
Sequence Analysis Tools
View WP_047092042.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
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Sequence
MTSPSNNSPVISARNLRLTLGEGDAAVEILRGIDLDVARGETLALLGPSGSGKSSLMSVL
SGLERATSGKLTVAGEDFTALNEDALAMARRGRIGIVLQAFHLLPTMTAVENVATPLELA
GEDGAWERATAELEAVGLGHRLTHYPAQLSGGEQQRVAIARAIVSRPPLIFADEPTGNLD
TNTGASVEDALFERRAETGATLIIITHDRKLAERCNRVVTLGDGLIATDTAAV
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory