GapMind for catabolism of small carbon sources

 

Protein WP_047094432.1 in Erythrobacter marinus HWDM-33

Annotation: NCBI__GCF_001013305.1:WP_047094432.1

Length: 354 amino acids

Source: GCF_001013305.1 in NCBI

Candidate for 12 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
4-hydroxybenzoate catabolism adh hi Alcohol dehydrogenase; EC 1.1.1.1; EC 1.1.1.4; EC 1.2.1.3 (characterized) 71% 98% 495.7 NADP-dependent isopropanol dehydrogenase; EC 1.1.1.80 37% 224.9
2'-deoxyinosine catabolism adh hi Alcohol dehydrogenase; EC 1.1.1.1; EC 1.1.1.4; EC 1.2.1.3 (characterized) 71% 98% 495.7 NADP-dependent isopropanol dehydrogenase; EC 1.1.1.80 37% 224.9
2-deoxy-D-ribose catabolism adh hi Alcohol dehydrogenase; EC 1.1.1.1; EC 1.1.1.4; EC 1.2.1.3 (characterized) 71% 98% 495.7 NADP-dependent isopropanol dehydrogenase; EC 1.1.1.80 37% 224.9
ethanol catabolism adh hi Alcohol dehydrogenase; EC 1.1.1.1; EC 1.1.1.4; EC 1.2.1.3 (characterized) 71% 98% 495.7 NADP-dependent isopropanol dehydrogenase; EC 1.1.1.80 37% 224.9
ethanol catabolism etoh-dh-nad hi Alcohol dehydrogenase; EC 1.1.1.1; EC 1.1.1.4; EC 1.2.1.3 (characterized) 71% 98% 495.7 NADP-dependent isopropanol dehydrogenase; EC 1.1.1.80 37% 224.9
L-threonine catabolism adh hi Alcohol dehydrogenase; EC 1.1.1.1; EC 1.1.1.4; EC 1.2.1.3 (characterized) 71% 98% 495.7 NADP-dependent isopropanol dehydrogenase; EC 1.1.1.80 37% 224.9
thymidine catabolism adh hi Alcohol dehydrogenase; EC 1.1.1.1; EC 1.1.1.4; EC 1.2.1.3 (characterized) 71% 98% 495.7 NADP-dependent isopropanol dehydrogenase; EC 1.1.1.80 37% 224.9
L-tryptophan catabolism adh hi Alcohol dehydrogenase; EC 1.1.1.1; EC 1.1.1.4; EC 1.2.1.3 (characterized) 71% 98% 495.7 NADP-dependent isopropanol dehydrogenase; EC 1.1.1.80 37% 224.9
D-sorbitol (glucitol) catabolism sdh lo L-iditol 2-dehydrogenase; EC 1.1.1.14 (characterized) 34% 97% 167.9 Alcohol dehydrogenase; EC 1.1.1.1; EC 1.1.1.4; EC 1.2.1.3 71% 495.7
xylitol catabolism xdhA lo L-iditol 2-dehydrogenase; EC 1.1.1.14 (characterized) 34% 97% 167.9 Alcohol dehydrogenase; EC 1.1.1.1; EC 1.1.1.4; EC 1.2.1.3 71% 495.7
D-xylose catabolism xdhA lo L-iditol 2-dehydrogenase; EC 1.1.1.14 (characterized) 34% 97% 167.9 Alcohol dehydrogenase; EC 1.1.1.1; EC 1.1.1.4; EC 1.2.1.3 71% 495.7
L-threonine catabolism tdh lo L-threonine 3-dehydrogenase (EC 1.1.1.103) (characterized) 30% 99% 134.4 Alcohol dehydrogenase; EC 1.1.1.1; EC 1.1.1.4; EC 1.2.1.3 71% 495.7

Sequence Analysis Tools

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MKAAIFVEPGRIVLGEKPVPDVGPLDALMKITTTTICGTDVHILKGEYPVEKGLTVGHEP
VGRIEKLGSAVYGFTEGQRVIAGAITPSGTSTASLCGFHAQCGGQEGHGWKAIGGWRFGN
TIDGAQAEYLLVPDAMANLAPIPDGLSDEQVLMCPDIMSTGFGGAESGKIRIGDTVAVFA
QGPIGLFATAGAYMMGATTIIGVESIPARMAMSRQMGATHVVDFSKVDPVDEIMKITDGR
GVDVAIEALGLQSTFEGALRVLRPGGTLSSLGVYSGDLTIPCDAFSAGLGDNHIVTTLCP
GGKERMRRLMATVASGRIDTKALVTHRFPLDSIVDAYELFANQRGGVLKVAITT

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory