Alignments for a candidate for fruI in Erythrobacter marinus HWDM-33

GapMind for catabolism of small carbon sources

Alignments for a candidate for fruI in Erythrobacter marinus HWDM-33

Align Phosphoenolpyruvate--protein phosphotransferase (EC 2.7.3.9) (characterized)
to candidate WP_047092185.1 AAV99_RS01420 phosphoenolpyruvate--protein phosphotransferase

Query= reanno::psRCH2:GFF3291
         (960 letters)



>NCBI__GCF_001013305.1:WP_047092185.1
          Length = 756

 Score =  276 bits (706), Expect = 4e-78
 Identities = 176/544 (32%), Positives = 281/544 (51%), Gaps = 19/544 (3%)

Query: 406 DQVNGIAASPGIAIGPVLVRKPQVIDYPKRGESPVIELQRLDAALDKVHADIGTLIDESQ 465
           DQ+ G+    G+AIG  +  +P+V       E    E +R+  A D++   I  +   ++
Sbjct: 180 DQIEGLTLVKGLAIGEAVFHQPRVQIEHTVAEDTEAERERVYLAFDRMREQIDNMTGHAE 239

Query: 466 VA---SIRDIFTTHQAMLKDPALREEVQVRLQKGLSAEAAWMEEIESAAQQQEALHDKLL 522
                    +  T++    D      +   +  GL+AEAA     +    +   + D LL
Sbjct: 240 FGVGGEHEQVLETYKMFAYDEGWSRRINEAIDSGLTAEAAIERVQQRTRMRMREISDPLL 299

Query: 523 AERAADLRDVGRRVLACLTG---VEAEQAPDEPYILVMDEVAPSDVATLNAQRVAGILTA 579
            +R  DL D+  R++  ++G     A++      ILV   + P+++   + +R+ G++  
Sbjct: 300 QDRMHDLEDLANRLIRIVSGQLGTAAQKGLTRDSILVAKNLGPAELLEYDKRRLKGVVLE 359

Query: 580 GGGATSHSAIIARALGIPAIVGAGPGVLGLARN-TLLLLDGERGELLVAPSGAQLEQARS 638
            G  T+H  I+ARA+G+P +VG    + GL R    +L+DG+ G + + P    L    +
Sbjct: 360 EGSLTAHVVIVARAMGVP-VVGRIRNLRGLVREGDEVLVDGDTGSVTLRPGPGVLTAFEA 418

Query: 639 ERAAREERKHLANERRMDAAVTRDGHPVEIAANIGAAGETPEAVAMGAEGIGLLRTELVF 698
             A   E+K    + R     TRDG  +E+  N G   +       GA+GIGL RTE  F
Sbjct: 419 RFARAREQKAAYAKLRDVEPFTRDGERIEVMMNAGLRDDVGMLAMTGADGIGLYRTEFQF 478

Query: 699 MNHSQAPNQATQEAEYRRVLEALEGRPLVVRTLDVGGDKPLPYWPMPA---EENPFLGVR 755
           +  S  P +  Q   YR VL+A +G+ ++ RT+D+GGDK LPY        +ENP +G R
Sbjct: 479 LVSSALPQRERQARLYRDVLDAAKGKRVIFRTVDIGGDKTLPYLTNEIGKQDENPAMGWR 538

Query: 756 GIRLSLQRPDILETQLRALLASADGRPLRIMFPMVGNIDEWRTAKAM----VDRLRV--- 808
            +RL+L+R  +++ Q RALL    GR L +MFPMV    E+  AKA+    +D LR    
Sbjct: 539 ALRLALEREGLMKVQARALLEGCAGRELNVMFPMVSEPWEFDAAKAVFEQQLDFLRKHKH 598

Query: 809 ELPVADLQVGIMIEIPSAALIAPVLAQEVDFFSIGTNDLTQYTLAIDRGHPTLSGQADGL 868
           +LP A ++ G M+E+PS A +   L  ++ F SIGTNDLTQ+  A DR +P L+ + D L
Sbjct: 599 QLPSA-IRYGAMLEVPSLAEMLDQLLPKLSFISIGTNDLTQFLFAADRANPMLAERYDWL 657

Query: 869 HPAVLRLIGMTVEAAHAHGKWVGVCGELAADALAVPLLVGLGVDELSVSARSIALVKARV 928
            PA+LR +   V++   +   +GVCGE+    L    L+GLG+  LS++   +  +K  V
Sbjct: 658 SPAILRFLDRVVKSCVGYPVDLGVCGEMGGRRLEALALIGLGIRRLSITPAGVGPIKELV 717

Query: 929 RELD 932
           R++D
Sbjct: 718 RKVD 721


Lambda     K      H
   0.317    0.134    0.384 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1308
Number of extensions: 55
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 960
Length of database: 756
Length adjustment: 42
Effective length of query: 918
Effective length of database: 714
Effective search space:   655452
Effective search space used:   655452
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources