Alignments for a candidate for nagF in Erythrobacter marinus HWDM-33

GapMind for catabolism of small carbon sources

Alignments for a candidate for nagF in Erythrobacter marinus HWDM-33

Align N-acetylglucosamine-specific PTS system, I, HPr, and IIA components (nagF) (characterized)
to candidate WP_047092185.1 AAV99_RS01420 phosphoenolpyruvate--protein phosphotransferase

Query= reanno::pseudo3_N2E3:AO353_04460
         (838 letters)



>NCBI__GCF_001013305.1:WP_047092185.1
          Length = 756

 Score =  255 bits (651), Expect = 8e-72
 Identities = 183/583 (31%), Positives = 283/583 (48%), Gaps = 19/583 (3%)

Query: 243 ALQALLNALSTAVNDDSHAAAPTPIAQRTRTAEAGVLNGVCAAPGL-VGGPLFQLAAIPL 301
           ALQ +   L+  +N+ +     T             + G+    GL +G  +F    + +
Sbjct: 146 ALQTVAMVLAELINNANLVDEETVGGLSEARTGQDQIEGLTLVKGLAIGEAVFHQPRVQI 205

Query: 302 PEDTGKHNAEEQLQALDRALEQVRSEIRETLSHAKKHKHTEEEQIFAAHLALLEDPALLE 361
            E T   + E + + +  A +++R +I     HA+     E EQ+   +     D     
Sbjct: 206 -EHTVAEDTEAERERVYLAFDRMREQIDNMTGHAEFGVGGEHEQVLETYKMFAYDEGWSR 264

Query: 362 AAIQSIDQGSAATHAWSQSIEAQCEVLQQLGNPLLAERANDLRDLRQRVLRALLGQ---D 418
              ++ID G  A  A  +  +     ++++ +PLL +R +DL DL  R++R + GQ    
Sbjct: 265 RINEAIDSGLTAEAAIERVQQRTRMRMREISDPLLQDRMHDLEDLANRLIRIVSGQLGTA 324

Query: 419 WHYDVPAGAIVAAHELTPSDLLQLSQQGVAGLCMAEGGATSHVAILARGKGLPCLVALSA 478
               +   +I+ A  L P++LL+  ++ + G+ + EG  T+HV I+AR  G+P +  +  
Sbjct: 325 AQKGLTRDSILVAKNLGPAELLEYDKRRLKGVVLEEGSLTAHVVIVARAMGVPVVGRIRN 384

Query: 479 SLLQQPQGQSVVLDADGGRLELTPDSQRLEQVAQAQREHLQRRERQQAQAH---TPAHTR 535
                 +G  V++D D G + L P    L      +    + RE++ A A        TR
Sbjct: 385 LRGLVREGDEVLVDGDTGSVTLRPGPGVLTAF---EARFARAREQKAAYAKLRDVEPFTR 441

Query: 536 DGLRIEVAANVASSNEAADALKGGADGVGLLRTEFLFVDRQTAPDEQEQRQAYQAVLDAM 595
           DG RIEV  N    ++       GADG+GL RTEF F+     P  + Q + Y+ VLDA 
Sbjct: 442 DGERIEVMMNAGLRDDVGMLAMTGADGIGLYRTEFQFLVSSALPQRERQARLYRDVLDAA 501

Query: 596 GDKSVIIRTIDVGGDKQLDYLPLPA---EANPVLGLRGIRMAQVRPELLDQQLRALLQVS 652
             K VI RT+D+GGDK L YL       + NP +G R +R+A  R  L+  Q RALL+  
Sbjct: 502 KGKRVIFRTVDIGGDKTLPYLTNEIGKQDENPAMGWRALRLALEREGLMKVQARALLEGC 561

Query: 653 PLQRCRILLPMVTEVDEL----LYIRQRLDALCAEL-ALTQRLELGVMIEVPAAALLAEQ 707
             +   ++ PMV+E  E         Q+LD L      L   +  G M+EVP+ A + +Q
Sbjct: 562 AGRELNVMFPMVSEPWEFDAAKAVFEQQLDFLRKHKHQLPSAIRYGAMLEVPSLAEMLDQ 621

Query: 708 LAEHADFLSIGTNDLSQYTLAMDRDHAGLAARVDALHPALLRLIAQTCIGAAKHQRWVGV 767
           L     F+SIGTNDL+Q+  A DR +  LA R D L PA+LR + +       +   +GV
Sbjct: 622 LLPKLSFISIGTNDLTQFLFAADRANPMLAERYDWLSPAILRFLDRVVKSCVGYPVDLGV 681

Query: 768 CGALASDPLATPVLIGLGISELSVSPPQVGEIKERVRQLDAAD 810
           CG +    L    LIGLGI  LS++P  VG IKE VR++D  +
Sbjct: 682 CGEMGGRRLEALALIGLGIRRLSITPAGVGPIKELVRKVDTKE 724


Lambda     K      H
   0.318    0.133    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1155
Number of extensions: 50
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 838
Length of database: 756
Length adjustment: 41
Effective length of query: 797
Effective length of database: 715
Effective search space:   569855
Effective search space used:   569855
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources