Alignments for a candidate for sdc in Erythrobacter marinus HWDM-33

GapMind for catabolism of small carbon sources

Alignments for a candidate for sdc in Erythrobacter marinus HWDM-33

Align The Na+-coupled dicarboxylate (succinate; malate; fumarate) transporter, SdcL (transports aspartate, α-ketoglutarate and oxaloacetate with low affinity). Km for succinate, ~6 (characterized)
to candidate WP_047094421.1 AAV99_RS12620 DASS family sodium-coupled anion symporter

Query= TCDB::Q65NC0
         (546 letters)



>NCBI__GCF_001013305.1:WP_047094421.1
          Length = 489

 Score =  395 bits (1015), Expect = e-114
 Identities = 201/484 (41%), Positives = 299/484 (61%), Gaps = 12/484 (2%)

Query: 51  PSYKTPQKIGLLLGPALFFAVLLFFFPEGLSYEGRMVLATTLWVAVWWITEAVPIPAASL 110
           PS  +P+ +GL  G A F A+L FF PEGL Y  R V A+   +A+WW TEA+P+P  +L
Sbjct: 10  PSEASPKLVGLAAGIAAFVAILTFFTPEGLDYPARFVAASGTMMAIWWATEAIPLPVTAL 69

Query: 111 LPIVLLPLTGA--LEGAAVTSSYGDPIVFLFLGGFLIALAMERWNLHKRIALNIISVVGT 168
           LPI++ PL G   +EGAA  + Y +PI+FLFLGGF++ALA+ER  LH+RIA+ I   +G 
Sbjct: 70  LPIIVFPLAGVAGIEGAA--APYANPIIFLFLGGFIVALAVERSELHRRIAMLIFRAMGK 127

Query: 169 STSRIVLGFMAATGFLSMWVSNTAAVMMMLPIGTAIIHQVSAVIKSERKDLAAEEAK--F 226
           S   +V GFM A   LSMW+SNT+  +M+ PI  ++     A++  E      ++AK  F
Sbjct: 128 SGKGLVAGFMLAAALLSMWISNTSTTLMLFPIAVSL-----ALLVGETSPGLTDKAKRDF 182

Query: 227 SKALIFSIGYAGTIGGLGTLIGTPPNIILAANIKKLYGVEVSFGGWMAFAVPVVVILLVA 286
             +L+  + Y  +IGG+ TL+GTP N  +   ++  Y +E+ F  WM   +PV +ILL  
Sbjct: 183 QISLLLGLAYGASIGGVATLVGTPTNAFMVGFLQSEYQIEIPFAQWMLIGIPVSIILLPL 242

Query: 287 VWLYLTKVAHPIKMKELPGGKELILEEKRKLGKMSFEETMVLLVFGFAAFMWVTRTFLWD 346
            W+ LT+  +P+     P  +E +  +   LG MS  E    ++F      WV R    D
Sbjct: 243 GWMLLTRFLYPVSFVSSPETREELARQYDALGAMSNSEKRTAMLFLLLVLGWVLRKPFAD 302

Query: 347 DK-IPGIDDTMIAIFAASLLFLIPSLNKGGRVLDWSVSKDLPWGILLLFGGGLALATGFK 405
              I G+ D  +A+ AA + F+IPS      ++ W  +K LPWG+L+LFGGGL+LA    
Sbjct: 303 ATGIEGLSDAGVAMAAALIAFVIPSGTDRHALVTWEDTKRLPWGVLILFGGGLSLAAALS 362

Query: 406 ETGLAEWIGGRLTVLDGFNFVVIVIISTALVLFLTEITSNTATATMILPVLASLALALNV 465
            TG+  W+G +L  L   N +++V+  T+LV+FLTE+TSN AT   +LPV+A+LA  L +
Sbjct: 363 ATGMTLWLGQQLAPLGAINHILLVVALTSLVIFLTELTSNVATTATLLPVVAALAFELGL 422

Query: 466 HPYALMVPAAMAANCAFMLPVGTPPNAIIFASGKLKISEMVRTGFVINIFTLILIVGAVF 525
            P  L+VP  +AA+CAFMLPV TPPNAI+F++G+++I +M+R GF +NI  ++L+     
Sbjct: 423 DPLILVVPVTIAASCAFMLPVATPPNAIVFSTGEVQIRDMMRAGFWLNIVAIVLVSIIAV 482

Query: 526 YILP 529
           +++P
Sbjct: 483 WLVP 486



 Score = 25.8 bits (55), Expect = 0.004
 Identities = 23/88 (26%), Positives = 37/88 (42%), Gaps = 15/88 (17%)

Query: 474 AAMAANCAFMLPVGTPPNA---------------IIFASGKLKISEMVRTGFVINIFTLI 518
           A  AA  AF++P GT  +A               I+F  G    + +  TG  + +   +
Sbjct: 315 AMAAALIAFVIPSGTDRHALVTWEDTKRLPWGVLILFGGGLSLAAALSATGMTLWLGQQL 374

Query: 519 LIVGAVFYILPHLWGVDLTVFPDNLKSN 546
             +GA+ +IL  +    L +F   L SN
Sbjct: 375 APLGAINHILLVVALTSLVIFLTELTSN 402


Lambda     K      H
   0.326    0.140    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 721
Number of extensions: 41
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 546
Length of database: 489
Length adjustment: 35
Effective length of query: 511
Effective length of database: 454
Effective search space:   231994
Effective search space used:   231994
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources