Alignments for a candidate for drdehyd-beta in Novosphingobium barchaimii LL02

GapMind for catabolism of small carbon sources

Alignments for a candidate for drdehyd-beta in Novosphingobium barchaimii LL02

Align 2-deoxy-D-ribose dehydrogenase β subunit (characterized)
to candidate WP_059152325.1 V474_RS15235 xanthine dehydrogenase family protein molybdopterin-binding subunit

Query= metacyc::MONOMER-20833
         (739 letters)



>NCBI__GCF_001046635.1:WP_059152325.1
          Length = 756

 Score =  224 bits (571), Expect = 1e-62
 Identities = 229/784 (29%), Positives = 329/784 (41%), Gaps = 101/784 (12%)

Query: 14  LSRRRFLAST-AVGALVIGF-------GLPLGAGRVQAATSAERGTQVPAFLEIRPDGTV 65
           ++RR  L    A G L +G+        LPL  G  + A  A        +++I  DG V
Sbjct: 3   VTRRGILVGALAGGGLALGYMLRPRRYPLPLAPGEGEYAFDA--------WIKIGSDGVV 54

Query: 66  RLLSPFMEGGQGTHTAMAQIVGEELDADPATFIVEAAPPGEAYV---------------- 109
            +  P +E GQG  T + Q+V  EL AD     VE AP    Y                 
Sbjct: 55  TVAVPQVEMGQGITTLIPQVVAHELGADWRQVAVEPAPVSGLYANLALAAKWAPLWMPLA 114

Query: 110 ---------------VMENGLRITGGSMSVRMSYPTMRRLGALARAMLLQAGAKQWGVPV 154
                            +    +T     +       R   A ARAML+QA A +W V  
Sbjct: 115 PSLADQPEDLLTRRWAQDRRFNVTADGTELAAYEGPARAAAASARAMLMQAAAARWKVSW 174

Query: 155 GELTTQPGRVVHAASGRSLGYGELASSALDMPVPDP----ASITLRDPSQFRWIGKPVK- 209
            E     G V H   G+ L +G LA  A     P P    A      P +F   G P++ 
Sbjct: 175 EECEAGDGFVTH--EGKRLPFGSLAEEAAAFSPPSPPPLRAEAMAERPGEFP-PGAPLRY 231

Query: 210 -RLDAYDKSTGKALYSIDLKVDNMLHAAVQHAP-RLGMTVGSLRNQSQVEGMKGVHSV-- 265
            RLD   K  G   ++ D+++  M+HAA+ HAP  L  ++  L +++ V+GM+G   +  
Sbjct: 232 PRLDLPAKVDGSWTFAGDVRLPGMVHAAIAHAPIGLNTSISGL-DEAAVKGMRGYRGIVR 290

Query: 266 -HVLPGA-VAVVAERWWHAKRAVEAIQVDWQEAA-ADSALRVMPADFSSDKHREFLAAQQ 322
                GA VA VA   W A+ A+  +   ++ AA ADS L     D + D+    + A  
Sbjct: 291 GMTNGGAWVAAVATDGWTAQAALTRMAPRFKVAAGADSDL----VDAAMDEALHTVDAAT 346

Query: 323 GPTRDDENEGDVAGALKGAKTQVEATYHNQYLNHAQLEPPSALARFNPDGTLEIWLPNQA 382
             T+ D        +L+G    ++  Y      HA +E  +A A F+ +G LE+W+  QA
Sbjct: 347 IHTQGDRE------SLRGG-LPLQLRYEVAPAIHAAIETAAAAAHFS-EGRLELWVGTQA 398

Query: 383 PDMFRADIAKRTGLDPAQITLHSPLLGGFFGRHFLYDSANPYPQAIALAKAVSRPIKLIW 442
           P+  R   A+  GL    + L+   +GG F R        P  QA  +AKA+ +P++L W
Sbjct: 399 PEAAREAAARAIGLQTRDVVLYPMAIGGSFDRRL---ENEPAAQAAVIAKAIGKPVQLTW 455

Query: 443 SREEEFLRDVLRPLAVVKFRAALDDKGLPV---AIEAVSAT-----------EGPTEALA 488
           SR EEF   + R        A +D +G      A  AV AT           + P +ALA
Sbjct: 456 SRREEFRAGLPRAPVAAVMGARVDAEGAIAGWRARIAVPATAREFGRRLLDGDAPFDALA 515

Query: 489 GKQGDKIDPTAVEGLSGKSYAIPNKRIAQIYVKGAPMLGYWRSVGNSLNDFFYESFLDEL 548
              G K D  AVEG +   Y IPN  +  +  K        R   ++   FF ESF+DEL
Sbjct: 516 AVAG-KADAMAVEG-AVPPYDIPNLVVDHVPAKIGLPTARMRGNAHAWTAFFNESFVDEL 573

Query: 549 ADKGGHDPYELRLHLLRDNPRLTTLLQAAGELSGGWKRGPFTAEDGTRRARGVAMASPFG 608
           A K   +P   R+ +L    RL   LQ    L+  W  G      G    +G+A      
Sbjct: 574 AHKASREPLSYRMAMLGKETRLAECLQRVAVLAQ-WGGG------GDASGQGIACHVMGE 626

Query: 609 SHTAAIAEVSIENGKVKVHDIWQAIDPGSIVNPAIVEAQVNGAVALGLSQTLLEEAVYVD 668
              A +A    +   V+V  +    D G IVN  I   Q+ G +  GL   L     Y  
Sbjct: 627 GRIAVVASARRDENGVRVDRLSAVADIGRIVNYDIARQQIEGGLVFGLGLALGCAVRYEG 686

Query: 669 GKPRARNYDLYPILAPAQMARVHVRVVESGEKMGGIGEPPLPAVAPAVANAVAQLTGQRV 728
           G+P + +     +   A    + V  ++S  +    GE  + AVAPAVANA+   TG R+
Sbjct: 687 GRPDSDSLGAMGLPKLADCPAIEVEFIDSKAQPADPGELGVAAVAPAVANALYSATGLRL 746

Query: 729 RSLP 732
           R LP
Sbjct: 747 RRLP 750


Lambda     K      H
   0.317    0.134    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1435
Number of extensions: 87
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 3
Number of HSP's successfully gapped: 2
Length of query: 739
Length of database: 756
Length adjustment: 40
Effective length of query: 699
Effective length of database: 716
Effective search space:   500484
Effective search space used:   500484
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources