Align cyclohexa-1,5-dienecarbonyl-CoA hydratase (EC 4.2.1.100) (characterized)
to candidate WP_059151871.1 V474_RS12920 enoyl-CoA hydratase
Query= BRENDA::D3RXI0 (252 letters) >NCBI__GCF_001046635.1:WP_059151871.1 Length = 255 Score = 108 bits (269), Expect = 1e-28 Identities = 75/248 (30%), Positives = 124/248 (50%), Gaps = 7/248 (2%) Query: 10 KDERVARIKIANPPV-NVLDMETMKEIISAIDEVEGVDVI---VFSGEG-KSFSAGAEIK 64 +++ +A I + P N L E + + +A E D + + +G G K+F AG ++K Sbjct: 10 REDGIAVITLNRPEQRNALTAEVREGLRAAWARFEADDALRIAILTGSGDKAFCAGGDLK 69 Query: 65 EHFPDKAPEMIRWFTQLIDKVLRCKAITVAAVKGFALGGGFELAIACDFVLASKNAKLGV 124 E R L + T+AAV G A GG+ +A ACD +AS AK + Sbjct: 70 EMVETGMAVPPRDMYALPYDTIELSKPTIAAVNGVAFAGGWMIAQACDLCVASTAAKFAI 129 Query: 125 PEITLAHYPPVAIALLPRMIGWKNAYELILTGEAITAERAFEIGLVNKVFEDENFEESVN 184 E+ + P A L+ MIG + E++LTG+ I A RA+EIGLVN++ + ++ Sbjct: 130 TEVKVGRGSPWAAPLI-HMIGQRIFMEIVLTGKPIDAARAYEIGLVNRLAQPGAVLDAAI 188 Query: 185 DFVNSLLEKSSVALRLTKKALLFSTEKEYLSLFDVINDVYLSQLVKSEDAVEGLKAFLEK 244 + +LE + ++++ ++ ++ STE + + S+DA EG +AF EK Sbjct: 189 ELAREVLEGAPLSVKAARETVMLSTEMGRSAALAAARSAH-EHTYNSQDAQEGPRAFAEK 247 Query: 245 RKPEWKGR 252 R+P W GR Sbjct: 248 RRPVWSGR 255 Lambda K H 0.318 0.136 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 137 Number of extensions: 7 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 252 Length of database: 255 Length adjustment: 24 Effective length of query: 228 Effective length of database: 231 Effective search space: 52668 Effective search space used: 52668 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory