Align Phosphate acetyltransferase; EC 2.3.1.8; Phosphotransacetylase (uncharacterized)
to candidate WP_066608841.1 V473_RS18950 bifunctional enoyl-CoA hydratase/phosphate acetyltransferase
Query= curated2:Q9X448 (316 letters) >NCBI__GCF_001046645.1:WP_066608841.1 Length = 473 Score = 365 bits (937), Expect = e-105 Identities = 191/294 (64%), Positives = 228/294 (77%) Query: 12 YDRLIAAARAEAPAVTIVAHPCDETSLGGAIEAAEMGLITPILVAPEAKIRNVAAEHRLD 71 + RLI R+ P T +AHPC ++ A AAE GLI PIL+ PEA+IR A + D Sbjct: 165 FRRLIEMTRSVEPVPTAIAHPCTTAAILAAAAAAEAGLIVPILIGPEARIRVAAKDAGKD 224 Query: 72 LGRREIVDVPHSHAAAAKAVALIREGRGELLMKGSLHTDELMHEVAASATGLRTQRRISH 131 +G ++DVPHSHAAAA+AV L+R G LLMKGSLHTDELM V +ATGLRT+RRISH Sbjct: 225 IGALRLIDVPHSHAAAARAVELVRSGEALLLMKGSLHTDELMGAVVPAATGLRTERRISH 284 Query: 132 VFVMDVPGHTDTLFITDAAINIFPDLEAKRDIVQNAIDLWVAIGLGEPRVAILSAVETVT 191 +++DVPGH L ITDAAINI P LE K DI++NAIDL IG+ P+VAILSAVETV Sbjct: 285 AYLLDVPGHPAPLIITDAAINIDPSLEDKADIIRNAIDLAHVIGIACPKVAILSAVETVN 344 Query: 192 AKIPSTIEAAALCKMAERGQITGGVLEGPLAFDNAIDQEAARIKGINSPVAGHAQILVVP 251 I ST++AAALCKMA+RGQI GGVL+GPLAFDNAI ++AA+ KGI SPVAG A++LVVP Sbjct: 345 PAIRSTLDAAALCKMADRGQIVGGVLDGPLAFDNAISEKAAKDKGIVSPVAGRAEVLVVP 404 Query: 252 DLEAGNMLAKNLTFLTHADAAGLVLGARVPIVLTSRADSVRTRLASCAVAALYA 305 +LEAGNMLAK LTFL ADAAG+VLGARVPI+LTSRADS+RT +ASCA+A L A Sbjct: 405 NLEAGNMLAKQLTFLGGADAAGVVLGARVPIILTSRADSLRTSVASCAIALLLA 458 Lambda K H 0.320 0.133 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 352 Number of extensions: 9 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 316 Length of database: 473 Length adjustment: 30 Effective length of query: 286 Effective length of database: 443 Effective search space: 126698 Effective search space used: 126698 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory