Alignments for a candidate for pimF in Sphingobium czechense LL01

GapMind for catabolism of small carbon sources

Alignments for a candidate for pimF in Sphingobium czechense LL01

Align 6-carboxyhex-2-enoyl-CoA hydratase (characterized)
to candidate WP_066608366.1 V473_RS18200 3-hydroxyacyl-CoA dehydrogenase

Query= metacyc::MONOMER-20678
         (699 letters)



>NCBI__GCF_001046645.1:WP_066608366.1
          Length = 681

 Score =  650 bits (1677), Expect = 0.0
 Identities = 342/693 (49%), Positives = 470/693 (67%), Gaps = 19/693 (2%)

Query: 1   MSEVVTRATQDQ-VAIVTVDSPPVNALSAAVRRGILENVNAAVADPAVQAIVLVCAGRTF 59
           MSEV T + +D  + ++ +D+PPVNAL    R  + E      AD  V+AIVL+C GRTF
Sbjct: 1   MSEVGTYSIEDGGIGVIEIDNPPVNALGIKTRLALDEGFRTFAADDRVKAIVLICGGRTF 60

Query: 60  IAGADITEFGKPPQPPALNDVIAALENSPKPTIAAIHGTALGGGLEVALGCHFRVAVKEA 119
            AGADI+EFG+P Q P L++V   +EN  KP +AAIHGTALGGG E+AL CH+R+AV  A
Sbjct: 61  FAGADISEFGQPQQEPGLHEVFDIIENGSKPVVAAIHGTALGGGYELALICHYRIAVPSA 120

Query: 120 KLGLPEVKLGLLPGAGGTQRLPRAVGPELAVQMIVGGSPIGAAEALKHGLVEEVVE--NL 177
           K+GLPEV LGLLPGAGGTQRLPR VG E A+ +I GG+P+ A +AL  G+++ V +  NL
Sbjct: 121 KVGLPEVNLGLLPGAGGTQRLPRIVGVETALDIITGGAPVPAKKALDLGMIDAVAQEGNL 180

Query: 178 VAGAVAFAKKVLAEKRPLRRLRDDDSKLAAAKADRSIFTNAVAAMTKKARGLEAPFACAD 237
              A+AFA+ ++   +PL+R+RD   K+A A+    I+   +    +  RG +AP+    
Sbjct: 181 REDAIAFARTIV--DKPLQRVRDRQDKVAPARGMPEIYEAYLKKNARAFRGFKAPYNIVR 238

Query: 238 AIGAAIDLP-FEEGLKKEREGFMKLVVSDQSKAQRYAFFAEREAAKVDGVPDGTKPRPVS 296
           AI A+ ++  F++G+++E+E F +LV S +S AQRY FFAERE AKV  VP  T   PV 
Sbjct: 239 AIEASAEIEDFDKGIEREKELFDELVTSTESAAQRYYFFAERETAKVPDVPRDTATIPVK 298

Query: 297 RVAIIGAGTMGGGIAMSFANAGIPVTLIETGEEQLKRGLGIMQKNWEATAARGGLPPDAP 356
            V +IGAGTMGGGI M+F N GIPVTL+E   E L RG+G+++KN+E TA +G +  D  
Sbjct: 299 SVGVIGAGTMGGGITMNFLNVGIPVTLVEMTREALDRGVGVIRKNYENTAKKGRMTADQV 358

Query: 357 AKRMALITGLVGLENVKDADLIIEAVFETMAVKKEVFTAVDAHAKPGAVLASNTSYLSID 416
            +RMALIT  +GLE + D DLIIEAV+E M VKK+VF  +D  AK GA+LASNTS+L +D
Sbjct: 359 EQRMALITPAIGLEALGDVDLIIEAVYEEMGVKKDVFGKLDKIAKQGAILASNTSFLDLD 418

Query: 417 EIAATTKRPQDVLGMHFFSPANVMKLCEIVRGAKTAPDALLTAVSIAKKIAKVPVVVGVC 476
           EIAA T RP  V+G+HFFSPANVM+L E+VRGAKT+ + + TA+++AKKI KVPV+ GVC
Sbjct: 419 EIAAATSRPDWVVGLHFFSPANVMRLLEVVRGAKTSKEVIATAMALAKKIGKVPVLSGVC 478

Query: 477 DGFVGNRMLAARSKQSEKLLFEGALPQQVDAVVTKFGMPMGPFAMGDLAGLDIGWRSRKD 536
            GF+ NR++A R  Q++ L+ EG  PQ +D  +  +G  MGPF M DL GLD+  R   +
Sbjct: 479 FGFIANRIMAKRGSQADALVLEGPTPQDIDQAIYDYGFAMGPFQMIDLVGLDVIGRDETE 538

Query: 537 RGIKSEIADALCEAGRFGQKTGKGYYKYEQGSRAPMPDPEVETLINDTLAKLGLKRRD-I 595
           R ++ +    L +A R GQK   GYY Y++  +A  P P     I D  A  G+K +  +
Sbjct: 539 RSVRGD----LVKADRLGQKKNGGYYDYDENRKA-TPSPVAAAAIADFAAFKGVKNKGAL 593

Query: 596 TDEEILERMVYPMINEGARILEEKIAARPSDIDVVWLYGYGWPIYRGGPMHYADSVGLKH 655
           + ++I+ R++YP++NEGAR+LEE +A R SD+DV  + GY WP+Y GGPM + D+VGL+ 
Sbjct: 594 SPDDIVARLLYPVVNEGARVLEEGVAIRASDVDVAAILGYNWPVYTGGPMFWGDTVGLQK 653

Query: 656 IAERLSAYAKATNDPSLEPAPLLARLAAEGKTF 688
           I + L+A         +EP+ LL   AA+G++F
Sbjct: 654 IVDGLTAM-------GIEPSRLLVEKAAKGESF 679


Lambda     K      H
   0.317    0.134    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1232
Number of extensions: 78
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 699
Length of database: 681
Length adjustment: 39
Effective length of query: 660
Effective length of database: 642
Effective search space:   423720
Effective search space used:   423720
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Rules (tab-delimited)
Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources