Protein WP_052591396.1 in Luteipulveratus mongoliensis MN07-A0370
Annotation: NCBI__GCF_001190945.1:WP_052591396.1
Length: 485 amino acids
Source: GCF_001190945.1 in NCBI
Candidate for 29 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| 4-hydroxybenzoate catabolism | adh | hi | Putative aldehyde dehydrogenase DhaS; EC 1.2.1.3 (characterized) | 49% | 95% | 436.8 | Potassium-activated aldehyde dehydrogenase, mitochondrial; K(+)-activated acetaldehyde dehydrogenase; K(+)-ACDH; EC 1.2.1.5 | 45% | 408.3 |
| 2'-deoxyinosine catabolism | adh | hi | Putative aldehyde dehydrogenase DhaS; EC 1.2.1.3 (characterized) | 49% | 95% | 436.8 | Potassium-activated aldehyde dehydrogenase, mitochondrial; K(+)-activated acetaldehyde dehydrogenase; K(+)-ACDH; EC 1.2.1.5 | 45% | 408.3 |
| 2-deoxy-D-ribose catabolism | adh | hi | Putative aldehyde dehydrogenase DhaS; EC 1.2.1.3 (characterized) | 49% | 95% | 436.8 | Potassium-activated aldehyde dehydrogenase, mitochondrial; K(+)-activated acetaldehyde dehydrogenase; K(+)-ACDH; EC 1.2.1.5 | 45% | 408.3 |
| ethanol catabolism | adh | hi | Putative aldehyde dehydrogenase DhaS; EC 1.2.1.3 (characterized) | 49% | 95% | 436.8 | Potassium-activated aldehyde dehydrogenase, mitochondrial; K(+)-activated acetaldehyde dehydrogenase; K(+)-ACDH; EC 1.2.1.5 | 45% | 408.3 |
| L-threonine catabolism | adh | hi | Putative aldehyde dehydrogenase DhaS; EC 1.2.1.3 (characterized) | 49% | 95% | 436.8 | Potassium-activated aldehyde dehydrogenase, mitochondrial; K(+)-activated acetaldehyde dehydrogenase; K(+)-ACDH; EC 1.2.1.5 | 45% | 408.3 |
| thymidine catabolism | adh | hi | Putative aldehyde dehydrogenase DhaS; EC 1.2.1.3 (characterized) | 49% | 95% | 436.8 | Potassium-activated aldehyde dehydrogenase, mitochondrial; K(+)-activated acetaldehyde dehydrogenase; K(+)-ACDH; EC 1.2.1.5 | 45% | 408.3 |
| L-tryptophan catabolism | adh | hi | Putative aldehyde dehydrogenase DhaS; EC 1.2.1.3 (characterized) | 49% | 95% | 436.8 | Potassium-activated aldehyde dehydrogenase, mitochondrial; K(+)-activated acetaldehyde dehydrogenase; K(+)-ACDH; EC 1.2.1.5 | 45% | 408.3 |
| L-fucose catabolism | aldA | med | NAD(P)+ L-lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized) | 43% | 94% | 404.4 | acetaldehyde dehydrogenase (EC 1.2.1.3) | 55% | 512.7 |
| L-rhamnose catabolism | aldA | med | NAD(P)+ L-lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized) | 43% | 94% | 404.4 | acetaldehyde dehydrogenase (EC 1.2.1.3) | 55% | 512.7 |
| L-threonine catabolism | aldA | med | NAD(P)+ L-lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized) | 43% | 94% | 404.4 | acetaldehyde dehydrogenase (EC 1.2.1.3) | 55% | 512.7 |
| L-phenylalanine catabolism | pad-dh | med | aldehyde dehydrogenase (NAD+) (EC 1.2.1.3) (characterized) | 46% | 93% | 403.7 | acetaldehyde dehydrogenase (EC 1.2.1.3) | 55% | 512.7 |
| 4-hydroxybenzoate catabolism | praB | med | 2-hydroxymuconate-6-semialdehyde dehydrogenase (EC 1.2.1.85) (characterized) | 44% | 95% | 384.4 | acetaldehyde dehydrogenase (EC 1.2.1.3) | 55% | 512.7 |
| L-tryptophan catabolism | praB | med | 2-hydroxymuconate-6-semialdehyde dehydrogenase (EC 1.2.1.85) (characterized) | 44% | 95% | 384.4 | acetaldehyde dehydrogenase (EC 1.2.1.3) | 55% | 512.7 |
| L-arginine catabolism | kauB | med | 4-guanidinobutyraldehyde dehydrogenase (EC 1.2.1.54) (characterized) | 44% | 94% | 368.2 | acetaldehyde dehydrogenase (EC 1.2.1.3) | 55% | 512.7 |
| L-arginine catabolism | puuC | med | 4-guanidinobutyraldehyde dehydrogenase (EC 1.2.1.54) (characterized) | 44% | 94% | 368.2 | acetaldehyde dehydrogenase (EC 1.2.1.3) | 55% | 512.7 |
| L-citrulline catabolism | puuC | med | 4-guanidinobutyraldehyde dehydrogenase (EC 1.2.1.54) (characterized) | 44% | 94% | 368.2 | acetaldehyde dehydrogenase (EC 1.2.1.3) | 55% | 512.7 |
| putrescine catabolism | puuC | med | 4-guanidinobutyraldehyde dehydrogenase (EC 1.2.1.54) (characterized) | 44% | 94% | 368.2 | acetaldehyde dehydrogenase (EC 1.2.1.3) | 55% | 512.7 |
| L-arginine catabolism | patD | med | aminobutyraldehyde dehydrogenase (EC 1.2.1.19) (characterized) | 42% | 94% | 357.1 | acetaldehyde dehydrogenase (EC 1.2.1.3) | 55% | 512.7 |
| L-citrulline catabolism | patD | med | aminobutyraldehyde dehydrogenase (EC 1.2.1.19) (characterized) | 42% | 94% | 357.1 | acetaldehyde dehydrogenase (EC 1.2.1.3) | 55% | 512.7 |
| putrescine catabolism | patD | med | aminobutyraldehyde dehydrogenase (EC 1.2.1.19) (characterized) | 42% | 94% | 357.1 | acetaldehyde dehydrogenase (EC 1.2.1.3) | 55% | 512.7 |
| L-arginine catabolism | gabD | med | 4-(gamma-glutamylamino)butanal dehydrogenase (EC 1.2.1.99) (characterized) | 42% | 94% | 354.8 | Potassium-activated aldehyde dehydrogenase, mitochondrial; K(+)-activated acetaldehyde dehydrogenase; K(+)-ACDH; EC 1.2.1.5 | 45% | 408.3 |
| L-citrulline catabolism | gabD | med | 4-(gamma-glutamylamino)butanal dehydrogenase (EC 1.2.1.99) (characterized) | 42% | 94% | 354.8 | Potassium-activated aldehyde dehydrogenase, mitochondrial; K(+)-activated acetaldehyde dehydrogenase; K(+)-ACDH; EC 1.2.1.5 | 45% | 408.3 |
| putrescine catabolism | gabD | med | 4-(gamma-glutamylamino)butanal dehydrogenase (EC 1.2.1.99) (characterized) | 42% | 94% | 354.8 | Potassium-activated aldehyde dehydrogenase, mitochondrial; K(+)-activated acetaldehyde dehydrogenase; K(+)-ACDH; EC 1.2.1.5 | 45% | 408.3 |
| L-tryptophan catabolism | nbaE | lo | 2-aminomuconic semialdehyde dehydrogenase; Aldehyde dehydrogenase 12; Aldehyde dehydrogenase family 8 member A1; EC 1.2.1.32 (characterized) | 38% | 95% | 322.4 | acetaldehyde dehydrogenase (EC 1.2.1.3) | 55% | 512.7 |
| L-lysine catabolism | patD | lo | aminobutyraldehyde dehydrogenase (EC 1.2.1.19) (characterized) | 39% | 98% | 312.8 | acetaldehyde dehydrogenase (EC 1.2.1.3) | 55% | 512.7 |
| L-arabinose catabolism | aldA | lo | lactaldehyde dehydrogenase (EC 1.2.1.22); D-glyceraldehyde dehydrogenase (NADP+) (EC 1.2.1.89) (characterized) | 36% | 98% | 284.3 | acetaldehyde dehydrogenase (EC 1.2.1.3) | 55% | 512.7 |
| D-xylose catabolism | aldA | lo | lactaldehyde dehydrogenase (EC 1.2.1.22); D-glyceraldehyde dehydrogenase (NADP+) (EC 1.2.1.89) (characterized) | 36% | 98% | 284.3 | acetaldehyde dehydrogenase (EC 1.2.1.3) | 55% | 512.7 |
| L-arginine catabolism | astD | lo | N-succinylglutamate 5-semialdehyde dehydrogenase 1; EC 1.2.1.71; Succinylglutamic semialdehyde dehydrogenase 1; SGSD 1 (uncharacterized) | 33% | 94% | 211.8 | acetaldehyde dehydrogenase (EC 1.2.1.3) | 55% | 512.7 |
| L-citrulline catabolism | astD | lo | N-succinylglutamate 5-semialdehyde dehydrogenase 1; EC 1.2.1.71; Succinylglutamic semialdehyde dehydrogenase 1; SGSD 1 (uncharacterized) | 33% | 94% | 211.8 | acetaldehyde dehydrogenase (EC 1.2.1.3) | 55% | 512.7 |
Sequence Analysis Tools
View WP_052591396.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
Sequence
MSKFEYAPAPESRAIVDLQSSYGLFINGEFVDGTGSSFKTINPATEEKLADVSEASADDV
DKAVKAARTAYTRVWSRMSGAERGKYVFRIARIMQERARELAVLESLDNGKPIKESRDVD
VPTAAAHFFYHAGWADKLEYAGLSPAGSPPPRPLGVVGQVIPWNFPLLMLAWKIAPALAT
GNTVVLKPAETTPLTALLFAEICQQADLPPGVVNIVTGAGATGQALVEHPDVDKIAFTGS
TDVGKAIARSIAGTRKRATLELGGKAANIVFEDAAIDQAVEGIVNGIFFNQGHVCCAGSR
LLVQESVADEVVRRLERRMKTLRVGDPLDKNTDVGAINSKAQLQRITELVDAGEAEGATR
WTVPCELPARGFWFAPTVFTDVSQTHRVATEEIFGPVLSVLTFRTPQEAVAKANNTPYGL
SAGIWTEKGSRILWMADQLKAGVVWANTFNRFDPTSPFGGYKESGYGREGGRHGLEAYLR
TGVDA
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory