GapMind for catabolism of small carbon sources

 

Protein WP_007700167.1 in Cronobacter universalis NCTC 9529

Annotation: NCBI__GCF_001277175.1:WP_007700167.1

Length: 231 amino acids

Source: GCF_001277175.1 in NCBI

Candidate for 2 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
glycerol catabolism glpF lo Nodulin-26 aquaporin and glycerol facilitator, NIP (de Paula Santos Martins et al. 2015). Transports NH3 5-fold better than water in Hg2+-sensitive fashion (characterized) 36% 78% 145.6 Aquaporin Z; Bacterial nodulin-like intrinsic protein 80% 381.3
glycerol catabolism TIPa lo Tonoplast intrinsic protein-a (transports water, urea, glycerol and gases (CO2 and NH3) (characterized) 34% 85% 105.5 Aquaporin Z; Bacterial nodulin-like intrinsic protein 80% 381.3

Sequence Analysis Tools

View WP_007700167.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MLRKCAAEFFGTFWLVFGGCGSAVLAAAYPELGIGFTGVALAFGLTVLTMAYAVGHISGG
HFNPAVTLGLWAGGRITFQNVIPYIISQVIGGIAAAGVLYAIASGKPGFDAVASGFAANG
YGEHSPDGYSLSAAILTELVLTAFFLLIIHGATDKNAPAKFAPLAIGLALTLIHLISIPV
TNTSVNPARSTAVAIFQGGWALRQLWVFWVVPMVGGVVGGLIYRFLLQSKD

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory