GapMind for catabolism of small carbon sources

 

Protein WP_007703136.1 in Cronobacter universalis NCTC 9529

Annotation: NCBI__GCF_001277175.1:WP_007703136.1

Length: 513 amino acids

Source: GCF_001277175.1 in NCBI

Candidate for 22 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
D-xylose catabolism xylG hi Xylose import ATP-binding protein XylG; EC 7.5.2.10 (characterized) 84% 100% 859.8 ATP binding protein of ABC transporter for pentoses, component of ABC sugar transporter that plays a role in the probiotic benefits through acetate production 46% 433.7
L-arabinose catabolism gguA med GguA aka ATU2347 aka AGR_C_4264, component of Multiple sugar (arabinose, xylose, galactose, glucose, fucose) putative porter (characterized) 48% 99% 436.4 Xylose import ATP-binding protein XylG; EC 7.5.2.10 84% 859.8
D-cellobiose catabolism mglA med GguA aka ATU2347 aka AGR_C_4264, component of Multiple sugar (arabinose, xylose, galactose, glucose, fucose) putative porter (characterized) 48% 99% 436.4 Xylose import ATP-binding protein XylG; EC 7.5.2.10 84% 859.8
D-galactose catabolism gguA med GguA aka ATU2347 aka AGR_C_4264, component of Multiple sugar (arabinose, xylose, galactose, glucose, fucose) putative porter (characterized) 48% 99% 436.4 Xylose import ATP-binding protein XylG; EC 7.5.2.10 84% 859.8
D-glucose catabolism mglA med GguA aka ATU2347 aka AGR_C_4264, component of Multiple sugar (arabinose, xylose, galactose, glucose, fucose) putative porter (characterized) 48% 99% 436.4 Xylose import ATP-binding protein XylG; EC 7.5.2.10 84% 859.8
lactose catabolism mglA med GguA aka ATU2347 aka AGR_C_4264, component of Multiple sugar (arabinose, xylose, galactose, glucose, fucose) putative porter (characterized) 48% 99% 436.4 Xylose import ATP-binding protein XylG; EC 7.5.2.10 84% 859.8
D-maltose catabolism mglA med GguA aka ATU2347 aka AGR_C_4264, component of Multiple sugar (arabinose, xylose, galactose, glucose, fucose) putative porter (characterized) 48% 99% 436.4 Xylose import ATP-binding protein XylG; EC 7.5.2.10 84% 859.8
sucrose catabolism mglA med GguA aka ATU2347 aka AGR_C_4264, component of Multiple sugar (arabinose, xylose, galactose, glucose, fucose) putative porter (characterized) 48% 99% 436.4 Xylose import ATP-binding protein XylG; EC 7.5.2.10 84% 859.8
trehalose catabolism mglA med GguA aka ATU2347 aka AGR_C_4264, component of Multiple sugar (arabinose, xylose, galactose, glucose, fucose) putative porter (characterized) 48% 99% 436.4 Xylose import ATP-binding protein XylG; EC 7.5.2.10 84% 859.8
D-ribose catabolism rbsA med ribose transport, ATP-binding protein RbsA; EC 3.6.3.17 (characterized) 46% 100% 431.8 Xylose import ATP-binding protein XylG; EC 7.5.2.10 84% 859.8
myo-inositol catabolism PS417_11890 med Inositol transport system ATP-binding protein (characterized) 46% 95% 411 Xylose import ATP-binding protein XylG; EC 7.5.2.10 84% 859.8
D-mannose catabolism HSERO_RS03640 med Ribose import ATP-binding protein RbsA; EC 7.5.2.7 (characterized, see rationale) 43% 96% 394.8 Xylose import ATP-binding protein XylG; EC 7.5.2.10 84% 859.8
D-galactose catabolism mglA med Galactose/methyl galactoside import ATP-binding protein MglA aka B2149, component of Galactose/glucose (methyl galactoside) porter (characterized) 41% 97% 389.8 Xylose import ATP-binding protein XylG; EC 7.5.2.10 84% 859.8
D-xylose catabolism xylK_Tm med Ribose import ATP-binding protein RbsA 1; EC 7.5.2.7 (characterized, see rationale) 45% 95% 384 Xylose import ATP-binding protein XylG; EC 7.5.2.10 84% 859.8
L-fucose catabolism HSERO_RS05250 med Ribose import ATP-binding protein RbsA; EC 7.5.2.7 (characterized, see rationale) 43% 96% 377.9 Xylose import ATP-binding protein XylG; EC 7.5.2.10 84% 859.8
myo-inositol catabolism iatA med Inositol transport ATP-binding protein IatA, component of The myoinositol (high affinity)/ D-ribose (low affinity) transporter IatP/IatA/IbpA. The structure of IbpA with myoinositol bound has been solved (characterized) 41% 97% 361.3 Xylose import ATP-binding protein XylG; EC 7.5.2.10 84% 859.8
D-fructose catabolism frcA med ABC-type sugar transport system, ATP-binding protein; EC 3.6.3.17 (characterized, see rationale) 43% 93% 357.1 Xylose import ATP-binding protein XylG; EC 7.5.2.10 84% 859.8
sucrose catabolism frcA med ABC-type sugar transport system, ATP-binding protein; EC 3.6.3.17 (characterized, see rationale) 43% 93% 357.1 Xylose import ATP-binding protein XylG; EC 7.5.2.10 84% 859.8
L-rhamnose catabolism rhaT' med RhaT, component of Rhamnose porter (Richardson et al., 2004) (Transport activity is dependent on rhamnokinase (RhaK; AAQ92412) activity (Richardson and Oresnik, 2007) This could be an example of group translocation!) (characterized) 41% 96% 355.9 Xylose import ATP-binding protein XylG; EC 7.5.2.10 84% 859.8
D-galactose catabolism BPHYT_RS16930 med Arabinose import ATP-binding protein AraG; EC 7.5.2.12 (characterized, see rationale) 41% 96% 349.7 Xylose import ATP-binding protein XylG; EC 7.5.2.10 84% 859.8
D-galactose catabolism ytfR lo galactofuranose ABC transporter putative ATP binding subunit (EC 7.5.2.9) (characterized) 38% 98% 334.7 Xylose import ATP-binding protein XylG; EC 7.5.2.10 84% 859.8
2'-deoxyinosine catabolism nupA lo RnsB, component of The (deoxy)ribonucleoside permease; probably takes up all deoxy- and ribonucleosides (cytidine, uridine, adenosine and toxic analogues, fluorocytidine and fluorouridine tested), but not ribose or nucleobases (characterized) 34% 99% 295.4 Xylose import ATP-binding protein XylG; EC 7.5.2.10 84% 859.8

Sequence Analysis Tools

View WP_007703136.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MPYLLEMKNITKAFGAVKAVDNVSLALAPGEVMSLCGENGSGKSTLMKVLCGIYPWGSYE
GEITFAGEPLTPAHIRDTERKGIAIIHQELALVKNLTVLENIFLGAEITRRGVLDYDAMT
VRCEKLLAQVSLFVSPHTRTGDLGLGQQQLVEIAKALNKQVRLLVLDEPTASLTEQETAL
LLDIIRDLQQHDIACIYISHKLNEVKAISDTICVIRDGKPVGTRPAAQMSEEEIIAMMVG
RELTALYPSEPHTPGDEILRVENLTAWHPVNRHIQRVSELSFSLRRGEILGIAGLVGAGR
TEAMQCLFGVWPGRWQGDIFIDGKPVTLRNCQQAIAHGIAMVPEDRKKDGIVPVMAVGQN
ITLAALDEFSGPLSALDAAAEQQCILDSLQRLKVKTASPMLAIGRLSGGNQQKAILARCL
LLNPRILILDEPTRGIDIGAKYEIYKLIHQLVQQGIAVIVISSELPEVLGLSDRVLVMHE
GRLKANLVNRHLTQEQVMEAALRSDRHVEKQSV

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory